Effects of Liver Fibrosis Progression on Tissue Relaxation Times in Different Mouse Models Assessed by Ultrahigh Field Magnetic Resonance Imaging.

Recently, clinical studies demonstrated that magnetic resonance relaxometry with determination of relaxation times T1 and T2⁎ may aid in staging and management of liver fibrosis in patients suffering from viral hepatitis and steatohepatitis. In the present study we investigated T1 and T2⁎ in differe...

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Publicado en:BioMed Research International Vol. 2017; pp. 1 - 11
Autores principales: Müller, Andreas, Hochrath, Katrin, Stroeder, Jonas, Hittatiya, Kanishka, Schneider, Günther, Lammert, Frank, Buecker, Arno, Fries, Peter
Formato: equations & formulas research tables/charts Journal Article
Publicado: Wiley-Blackwell 1/18/2017
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 1/18/2017
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2017/8720367
        120792653
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        atl: Effects of Liver Fibrosis Progression on Tissue Relaxation Times in Different Mouse Models Assessed by Ultrahigh Field Magnetic Resonance Imaging.
      aug:
        au:
          Müller, Andreas
          Hochrath, Katrin
          Stroeder, Jonas
          Hittatiya, Kanishka
          Schneider, Günther
          Lammert, Frank
          Buecker, Arno
          Fries, Peter
        affil: Clinic for Diagnostic and Interventional Radiology, Saarland University Medical Center, Kirrberger Str. 100, Bdg. 50.1, 66421 Homburg, Germany
      sug:
        subj:
          Magnetic Resonance Imaging Methods
          Liver Diseases Physiopathology
          Animal Studies
          Mice
          Liver Diseases Diagnosis
          Fibrosis
          Tissue Physiology
          Relaxation
          Carbon Compounds, Inorganic Adverse Effects
          Cholangitis, Sclerosing
          Liver Cirrhosis
          Molecular Biology
          Liver Anatomy and Histology
          Temperature
          Descriptive Statistics
          Funding Source
      ab: Recently, clinical studies demonstrated that magnetic resonance relaxometry with determination of relaxation times T1 and T2⁎ may aid in staging and management of liver fibrosis in patients suffering from viral hepatitis and steatohepatitis. In the present study we investigated T1 and T2⁎ in different models of liver fibrosis to compare alternate pathophysiologies in their effects on relaxation times and to further develop noninvasive quantification methods of liver fibrosis. MRI was performed with a fast spin echo sequence for measurement of T1 and a multigradient echo sequence for determination of T2⁎. Toxic liver fibrosis was induced by injections of carbon tetrachloride (1.4 mL CCl4 per kg bodyweight and week, for 3 or 6 weeks) in BALB/cJ mice. Chronic sclerosing cholangitis was mimicked using the ATP-binding cassette transporter B4 knockout (Abcb4 -/-) mouse model. Untreated BALB/cJ mice served as controls. To assess hepatic fibrosis, we ascertained collagen contents and fibrosis scores after Sirius red staining. T1 and T2⁎ correlate differently to disease severity and etiology of liver fibrosis. T2⁎ shows significant decrease correlating with fibrosis in CCl4 treated animals, while demonstrating significant increase with disease severity in Abcb4 -/- mice. Measurements of T1 and T2⁎ may therefore facilitate discrimination between different stages and causes of liver fibrosis.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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