Role of Cytosolic Calcium Diffusion in Murine Cardiac Purkinje Cells.
Cardiac Purkinje cells (PCs) are morphologically and electrophysiologically different from ventricular myocytes and, importantly, exhibit distinct calcium (Ca2+) homeostasis. Recent studies suggest that PCs are more susceptible to action potential (AP) abnormalities than ventricular myocytes; howeve...
| Publicado en: | Clinical Medicine Insights: Cardiology Vol. 10; pp. 17 - 27 |
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| Autores principales: | , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
2016 Supplement 1
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121030164&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121030164 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11795468 B3KL jtl: Clinical Medicine Insights: Cardiology issn: 11795468 maglogo: Y pubinfo: dt: 2016 Supplement 1 vid: 10 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 121030164 121030164 121030164 10.4137/CMC.S39705 121030164 ppf: 17 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Role of Cytosolic Calcium Diffusion in Murine Cardiac Purkinje Cells. aug: au: Limbu, Bijay Shah, Kushal Weinberg, Seth H. Deo, Makarand affil: Department of Engineering, Norfolk State University, Norfolk, VA, USA sug: subj: Diffusion Action Potentials Role Playing Calcium Cells Experimental Studies Comparative Studies Study Design Probability ab: Cardiac Purkinje cells (PCs) are morphologically and electrophysiologically different from ventricular myocytes and, importantly, exhibit distinct calcium (Ca2+) homeostasis. Recent studies suggest that PCs are more susceptible to action potential (AP) abnormalities than ventricular myocytes; however, the exact mechanisms are poorly understood. In this study, we utilized a detailed biophysical mathematical model of a murine PC to systematically examine the role of cytosolic Ca2+diffusion in shaping the AP in PCs. A biphasic spatiotemporal Ca2+diffusion process, as recorded experimentally, was implemented in the model. In this study, we investigated the role of cytosolic Ca2+dynamics on AP and ionic current properties by varying the effective Ca2+diffusion rate. It was observed that AP morphology, specifically the plateau, was affected due to changes in the intracellular Ca2+dynamics. Elevated Ca2+concentration in the sarcolemmal region activated inward sodium--Ca2+exchanger (NCX) current, resulting in a prolongation of the AP plateau at faster diffusion rates. Artificially clamping the NCX current to control values completely reversed the alterations in the AP plateau, thus confirming the role of NCX in modifying the AP morphology. Our results demonstrate that cytosolic Ca2+diffusion waves play a significant role in shaping APs of PCs and could provide mechanistic insights in the increased arrhythmogeneity of PCs. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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