Differential Effects of Resveratrol on the Expression of Brain-Derived Neurotrophic Factor Transcripts and Protein in the Hippocampus of Rat Brain.
Background: The induction of brain-derived neurotrophic factor (BDNF) expression in the hippocampus has shown to play a role in the beneficial effects of resveratrol (RSV) on the learning and memory. The BDNF gene has a complicated structure with eight 5' noncoding exons (I-IXa), each of which can s...
| Publicado en: | Iranian Journal of Medical Sciences Vol. 42; no. 1; pp. 32 - 40 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Shiraz University of Medical Sciences
Jan2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121048601&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121048601 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 02530716 8TQH jtl: Iranian Journal of Medical Sciences issn: 02530716 maglogo: N pubinfo: dt: Jan2017 vid: 42 iid: 1 pid: 65846 pub: Shiraz University of Medical Sciences place: Shiraz, <Blank> artinfo: ui: 121048601 121048601 121048601 121048601 ppf: 32 ppct: 8 formats: tig: atl: Differential Effects of Resveratrol on the Expression of Brain-Derived Neurotrophic Factor Transcripts and Protein in the Hippocampus of Rat Brain. aug: au: Shojaei, Shahla Panjehshahin, Mohammad Reza Shafiee, Sayed Mohammad Khoshdel, Zahra Borji, Mohammad Ghasempour, Ghasem Owji, Ali Akbar affil: Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran sug: subj: Resveratrol Pharmacodynamics Phytoestrogens Pharmacodynamics Brain-Derived Neurotrophic Factor Gene Expression Hippocampus Anatomy and Histology Animal Studies Rats Pregnancy Embryo Learning Drug Effects Memory Drug Effects Resveratrol Administration and Dosage Oral Medicine Female Gestational Age Dissection Reverse Transcriptase Polymerase Chain Reaction Blotting, Western Female ab: Background: The induction of brain-derived neurotrophic factor (BDNF) expression in the hippocampus has shown to play a role in the beneficial effects of resveratrol (RSV) on the learning and memory. The BDNF gene has a complicated structure with eight 5' noncoding exons (I-IXa), each of which can splice to a common coding exon (IX) to form a functional transcript. Estrogens increase levels of BDNF transcripts in the hippocampus of rats. The aim of this study was to evaluate the effects of the phytoestrogen, RSV, on the splicing pattern of BDNF transcripts and on the pro-BDNF protein in the hippocampi of mother rats and their embryos. Methods: RSV (60 or 120 mg/kg BW/day) was administered orally to pregnant rats from days 1 to 20 of gestation. Hippocampi of adults and embryos were dissected 24 h after the last administration of RSV. Extracts from hippocampi were subject to quantitative (q) RT-PCR and Western blotting to assess splicing pattern of the BDNF transcripts and levels of pro-BDNF protein, respectively. Results: RSV (120 mg/kg BW/day) caused a statistically significant increase in the expression levels of BDNF exons III, IV and IX, but not the exon I in the hippocampi of adult rats (P=0.05). Levels of pro-BDNF protein remained unchanged in the hippocampal tissues from both adult and embryonic rats treated by RSV (60 or 120 mg/kg BW/day). Conclusion: Our results showed that RSV differentially activates promoters of the BDNF gene in the hippocampus of pregnant rats, but fails to affect the pro-BDNF level neither in adult nor in the embryonic hippocampal tissues. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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