Increased Circulating Endothelial Microparticles Associated with PAK4 Play a Key Role in Ventilation-Induced Lung Injury Process.
Inappropriate mechanical ventilation (MV) can result in ventilator-induced lung injury (VILI). Probing mechanisms of VILI and searching for effective methods are current areas of research focus on VILI. The present study aimed to probe into mechanisms of endothelial microparticles (EMPs) in VILI and...
| Publicado en: | BioMed Research International Vol. 2017; pp. 1 - 11 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/5/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121122341&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121122341 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/5/2017 vid: 2017 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 121122341 121122341 121122341 10.1155/2017/4902084 121122341 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Increased Circulating Endothelial Microparticles Associated with PAK4 Play a Key Role in Ventilation-Induced Lung Injury Process. aug: au: Pan, Shuming Fei, Aihua Jing, Lihong Zhang, Xiangyu Gao, Chengjin affil: Emergency Department, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China sug: subj: Endothelium Cellular Structures Analysis Ventilator-Induced Lung Injury Prevention and Control Respiration, Artificial Adverse Effects Protein Kinases Antiinflammatory Agents Administration and Dosage Animal Studies Mice Cytoskeletal Proteins Antagonists and Inhibitors Models, Biological Tidal Volume Pulmonary Alveoli Neutrophils Cell Communication Respiratory Function Tests Fluorescent Antibody Technique Microscopy Methods Blotting, Western Staining and Labeling Bronchoalveolar Lavage Descriptive Statistics Data Analysis Software Two-Way Analysis of Variance T-Tests Post Hoc Analysis P-Value Edema Drug Therapy Inflammation Ventilator-Induced Lung Injury Complications Tissue Culture Techniques Blood Analysis Funding Source ab: Inappropriate mechanical ventilation (MV) can result in ventilator-induced lung injury (VILI). Probing mechanisms of VILI and searching for effective methods are current areas of research focus on VILI. The present study aimed to probe into mechanisms of endothelial microparticles (EMPs) in VILI and the protective effects of Tetramethylpyrazine (TMP) against VILI. In this study, C57BL/6 and TLR4KO mouse MV models were used to explore the function of EMPs associated with p21 activated kinases-4 (PAK-4) in VILI. Both the C57BL/6 and TLR4 KO groups were subdivided into a mechanical ventilation (MV) group, a TMP + MV group, and a control group. After four hours of high tidal volume (20 ml/kg) MV, the degree of lung injury and the protective effects of TMP were assessed. VILI inhibited the cytoskeleton-regulating protein of PAK4 and was accompanied by an increased circulating EMP level. The intercellular junction protein of β-catenin was also decreased accompanied by a thickening alveolar wall, increased lung W/D values, and neutrophil infiltration. TMP alleviated VILI via decreasing circulating EMPs, stabilizing intercellular junctions, and alleviating neutrophil infiltration. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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