Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway.
The mRNA expression of CAPN2 was upregulated in CRPC cells (DU145 and PC3) than that in non-CRPC cells. Silencing CAPN2 expression could inhibit DU145 and PC3 cells proliferation by cell cycle arrest at G1 phase. Knockdown of CPAN2 level suppressed the migration and invasion capacity of CRPC cells b...
| Publicado en: | BioMed Research International Vol. 2017; pp. 1 - 11 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/9/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121197463&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121197463 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/9/2017 vid: 2017 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 121197463 121197463 121197463 10.1155/2017/2593674 121197463 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Silencing CAPN2 Expression Inhibited Castration-Resistant Prostate Cancer Cells Proliferation and Invasion via AKT/mTOR Signal Pathway. aug: au: Li, Pu Miao, Chenkui Liang, Chao Shao, Pengfei Wang, Zengjun Li, Jie affil: State Key Laboratory of Reproductive Medicine and Department of Urology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, China sug: subj: Prostatic Neoplasms, Castration-Resistant Neoplasm Invasiveness Cell Cycle RNA Physiology Gene Expression Intracellular Signaling Peptides and Proteins Signal Transduction Human Male Middle Age Aged Descriptive Statistics Data Analysis Software P-Value T-Tests Cell Culture Techniques Blotting, Western Polymerase Chain Reaction Cytological Techniques Immunohistochemistry Funding Source Middle Aged: 45-64 years Aged: 65+ years Male ab: The mRNA expression of CAPN2 was upregulated in CRPC cells (DU145 and PC3) than that in non-CRPC cells. Silencing CAPN2 expression could inhibit DU145 and PC3 cells proliferation by cell cycle arrest at G1 phase. Knockdown of CPAN2 level suppressed the migration and invasion capacity of CRPC cells by reducing matrix metalloproteinase-2 (MMP-2) and MMP-9 activation, as well as repressing the phosphorylation protein expression of AKT and mTOR. In addition, we found that the expression of CAPN2 was elevated in Pca tissues than that in normal control tissues. Therefore, we showed the important roles of CAPN2 in the development and progression in CRPC cells, suggesting a new therapeutic intervention for treating castration-resistant prostate cancer patients. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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