In vitro and in vivo activity of EDTA and antibacterial agents against the biofilm of mucoid Pseudomonas aeruginosa.
Purpose: Refractory infection caused by bacterial biofilm is an important clinical problem. Pseudomonas aeruginosa is a common pathogen responsible for persistent and chronic biofilm infections. We aimed to explore the in vitro and in vivo activity of ethylenediamine tetraacetic acid (EDTA) in combi...
| Published in: | Infection Vol. 45; no. 1; pp. 23 - 32 |
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| Main Authors: | , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Springer Nature
Feb2017
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121238082&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121238082 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Feb2017 vid: 45 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 121238082 121238082 121238082 10.1007/s15010-016-0905-z 121238082 ppf: 23 ppct: 9 formats: fmt: @attributes: type: P tig: atl: In vitro and in vivo activity of EDTA and antibacterial agents against the biofilm of mucoid Pseudomonas aeruginosa. aug: au: Liu, Zhenqiu Lin, Yaying Lu, Qi Li, Fang Yu, Jialin Wang, Zhengli He, Yu Song, Chao affil: Department of Neonatology , Maternal and Child Health Hospital of Xiamen City , Xiamen China sug: subj: Biofilms Bacterial Infections Prevention and Control Pseudomonas Infections Therapy Descriptive Statistics Ampicillin Pharmacodynamics Ciprofloxacin Pharmacodynamics Gentamicins Guinea Pigs Microbial Culture and Sensitivity Tests Stem Cells In Vitro Studies In Vivo Studies Edetic Acid Pseudomonas Infections Physiopathology Recurrence Data Analysis Software Animal Studies Lung Diseases Analysis of Variance ab: Purpose: Refractory infection caused by bacterial biofilm is an important clinical problem. Pseudomonas aeruginosa is a common pathogen responsible for persistent and chronic biofilm infections. We aimed to explore the in vitro and in vivo activity of ethylenediamine tetraacetic acid (EDTA) in combination with antibacterial agents against mucoid P. aeruginosa biofilm. Methods: The minimal inhibitory concentration (MIC) and minimal bactericidal concentration of ciprofloxacin, gentamicin, and ampicillin alone or with EDTA against P. aeruginosa were determined in vitro. Extracellular polysaccharides (EPS) and structural parameters of the biofilm were monitored. P. aeruginosa was aerosolized and delivered into the lungs of guinea pigs, which were treated with ciprofloxacin with or without EDTA. The colony-forming units (CFUs) of P. aeruginosa were determined from the lungs. Results: EDTA reduced the MIC of ciprofloxacin and ampicillin by about 30-fold and that of gentamicin by twofold. EDTA reduced the biofilm EPS and the proportion of viable bacteria. The thickness, average diffusion distance, and textural entropy of EDTA-treated biofilm were significantly decreased. EDTA plus antibiotics reduced the colony counting from 10 to 10 CFU/mL. In vivo, EDTA plus ciprofloxacin had a significantly lower mean CFU/g of lung tissue (EDTA + ciprofloxacin 1.3 ± 0.19; EDTA 4.4 ± 0.57; ciprofloxacin 4.2 ± 0.47), and lung lesions were less severe compared with the single treatment groups. Conclusions: EDTA can destroy the biofilm structures of mucoid P. aeruginosa in vitro. Moreover, EDTA and ciprofloxacin had a significant bactericidal effect against biofilm in vivo. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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