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Prevalence of Helicobacter pylori among patients with different gastrointestinal disorders in Saudi Arabia.

Background: Helicobacter pylori is an important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. The present study was carried out to determine the relationship between this organism with different gastrointestinal ailments. Methods: 150...

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Published in:Medical Journal of Indonesia Vol. 25; no. 4; pp. 214 - 221
Main Author: Alhussaini, Mohammed S.
Format: pictorial research tables/charts Journal Article
Published: Universitas Indonesia, Faculty of Medicine Dec2016
Online Access:View this record in EBSCOhost
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      jtl: Medical Journal of Indonesia
      issn: 08531773
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      dt: Dec2016
      vid: 25
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      pub: Universitas Indonesia, Faculty of Medicine
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        10.13181/mji.v25i4.1442
        121239467
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      tig:
        atl: Prevalence of Helicobacter pylori among patients with different gastrointestinal disorders in Saudi Arabia.
      aug:
        au: Alhussaini, Mohammed S.
        affil: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Saudi Arabia
      sug:
        subj:
          Helicobacter Pylori
          Stomach Neoplasms
          Peptic Ulcer
          Duodenal Ulcer
          Prevalence
          Saudi Arabia
          Outpatients
          Data Collection
          Sampling Methods
          Sensitivity and Specificity
      ab:
        Background: Helicobacter pylori is an important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. The present study was carried out to determine the relationship between this organism with different gastrointestinal ailments. Methods: 150 outpatients referrals to Saudi Arabian Medical City, Riyadh, Kingdom of Saudi Arabia was recruited in January to June 2015. Each patient was subjected to endoscopic examination. Biopsy specimens were taken from the stomach for rapid urease test and culture. Suspected H. pylori colonies were subjected to colony morphology identification, microscopical examination and biochemical reactions. The samples were also subjected to PCR to detect ureA subunit of urease gene. Results: The endoscopic examination of patients revealed normal, gastric ulcer, duodenal ulcer, gastritis, and gastric cancer with a rate of 20.7%, 20%, 24%, 33.3%, and 2%, respectively. Direct smear exam revealed that 52% of patients were H. pylori positive while culture and rapid urease test showed a prevalence of 71.33%. Fifty four biopsies (36%) were urease positive after 1 hour at room temperature, 39 (62%) after 1 hour incubation at 37°C and 14 (71.33%) after 24 hours incubation. Isolated H. pylori showed that they were catalase, oxidase, and urease positive. PCR results showed 411-bp fragment, which is indicative for the ureA subunit of urease gene. Conclusion: The prevalence of H. pylori infection was high among tested population. Strong association between H. pylori and duodenal ulcer was noticed. A 411-bp fragment indicative of the ureA subunit of urease gene was detected in all the tested isolates.
        Latar belakang: Helicobacter pylori adalah patogen gastrointestinalyang pentingyang berkaitan dengan gastritis, ulkus peptikum, dan peningkatan risiko kanker lambung. Studi ini bertujuan mencari hubungan patogen ini dengan berbagai kelainan gastrointestinal. Metode: 150 pasien rawatjalanyang dirujuk ke SaudiArabian Medical City, Riyadh, Arab Saudi diikutsertakan pada bulan Januari sampai Juni 2015. Setiap pasien menjalani endoskopi dan biopsi diambil spesimennya untuk pemeriksaan urease dan kultur. Koloniyang dicurigai sebagai H. pylori dilakukan identifikasi morfologi, pemeriksaan mikroskopik dan biokimia. Dilakukanjuga pemeriksaan PCR untuk mendeteksigen urease subunit ureA. Hasil: Hasil endoskopibervariasi darinormal,ulkusgaster,ulkus duodenum, gastritis, dan kanker lambung dengan prevalensi masing-masing 20.7%, 20%, 24%, 33,3%, dan 2%. Pemeriksaan pewarnaan langsung menunjukkan 52% pasien positif H. pylori, sedangkan kultur dan uji cepat menghasilkan prevalensi 71,33%. Lima puluh empat biopsi (36%) menunjukkan urease positif setelah satujam pada suhu ruang, 39 spesimen (62%) setelah satujam inkubasi pada 37°C, dan 14 spesimen (71,33%) setelah inkubasi 24 jam. IsolatH. pylori memperlihatkan hasil positif katalase, oksidase, dan urease. Hasil PCR menunjukkan fragment411-bp,yang sesuai dengan gen urease subunitureA. Kesimpulan: Prevalensi H. pylori cukup tinggi pada populasi yang diteliti. Terdapat hubungan kuat antara H. pyori dengan ulkus duodenum. Fragmen 411-bp merupakan indikator adanya gen urease subunit ureA.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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