An association between ABO group and HLA antibody detection.
Background: US blood centers can screen female plateletpheresis donors with a history of one or more pregnancies for both Class I and Class II anti-HLA antibodies using one of two platforms. One is a flow-based assay that yields a quantitative result and the other an enzyme-linked immunosorbent assa...
| Published in: | Transfusion Vol. 57; no. 2; pp. 313 - 319 |
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| Main Authors: | , , , , , , , |
| Format: | research Journal Article |
| Published: |
Wiley-Blackwell
Feb2017
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121248713&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121248713 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00411132 1YB jtl: Transfusion issn: 00411132 maglogo: Y pubinfo: dt: Feb2017 vid: 57 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 121248713 121248713 NLM27807878 121248713 10.1111/trf.13901 NLM27807878 121248713 ppf: 313 ppct: 6 formats: tig: atl: An association between ABO group and HLA antibody detection. aug: au: Dunbar, Nancy M. Yazer, Mark H. Bravo, Marjorie D. Kamel, Hany T. Gorlin, Jed Norris, Philip J. Williamson, Phillip C. Vassallo, Ralph R. affil: Department of Pathology and Laboratory Medicine, Dartmouth‐Hitchcock Medical Center, Lebanon New Hampshire sug: subj: ABO Blood-Group System Blood Isoantibodies Blood Reproductive History HLA Antigens Plateletpheresis Pregnancy Female Adult Human Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adult: 19-44 years Female ab: Background: US blood centers can screen female plateletpheresis donors with a history of one or more pregnancies for both Class I and Class II anti-HLA antibodies using one of two platforms. One is a flow-based assay that yields a quantitative result and the other an enzyme-linked immunosorbent assay (ELISA) that yields either a positive or a negative result (above or below cutoff).Study Design and Methods: The results of HLA antibody screening tests were analyzed by donor ABO group. Results from large and small American blood collection centers using both platforms were analyzed. Positivity rates were compared by chi-square test and the results stratified by parity using the Mann-Whitney test.Results: No differences in parity were noted among donors of different ABO groups, but a significantly higher rate of HLA antibody positivity was observed among group O donors for the ELISA (31% of group O donors vs. 21% of non-group O donors, p < 0.0001). The higher rate of positivity was primarily due to Class I reactivity. This difference in antibody frequency was not observed at centers using the flow-based assay.Conclusion: Centers using the ELISA may have a higher rate of permanent deferral from plateletpheresis donation among group O female donors. Although the reasons for the higher rate of reactivity on Class I ELISA testing are unknown, this could result from test system characteristics or differences in group O donor antibody strength or specificity. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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