Creatine CEST MRI for Differentiating Gliomas with Different Degrees of Aggressiveness.

Purpose: Creatine (Cr) is a major metabolite in the bioenergetic system. Measurement of Cr using conventional MR spectroscopy (MRS) suffers from low spatial resolution and relatively long acquisition times. Creatine chemical exchange saturation transfer (CrCEST) magnetic resonance imaging (MRI) is a...

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Publicado en:Molecular Imaging & Biology Vol. 19; no. 2; pp. 225 - 233
Autores principales: Cai, Kejia, Tain, Rong-Wen, Zhou, Xiaohong, Damen, Frederick, Scotti, Alessandro, Hariharan, Hari, Poptani, Harish, Reddy, Ravinder, Zhou, Xiaohong Joe, Damen, Frederick C, Scotti, Alessandro M
Formato: diagnostic images pictorial research tables/charts Journal Article
Publicado: Springer Nature Apr2017
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Springer Nature
      place: New York, New York
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        atl: Creatine CEST MRI for Differentiating Gliomas with Different Degrees of Aggressiveness.
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        au:
          Cai, Kejia
          Tain, Rong-Wen
          Zhou, Xiaohong
          Damen, Frederick
          Scotti, Alessandro
          Hariharan, Hari
          Poptani, Harish
          Reddy, Ravinder
          Zhou, Xiaohong Joe
          Damen, Frederick C
          Scotti, Alessandro M
        affil: Department of Radiology and the Center for MR Research, College of Medicine , University of Illinois at Chicago , Chicago USA
      sug:
        subj:
          Magnetic Resonance Imaging Methods
          Creatine
          Glioma Pathology
          Cell Differentiation
          Brain Neoplasms Pathology
          Image Processing, Computer Assisted
          Animal Studies
          Neoplasm Invasiveness
          Signal Processing, Computer Assisted
          Rats
          Models, Biological
          Funding Source
      ab: Purpose: Creatine (Cr) is a major metabolite in the bioenergetic system. Measurement of Cr using conventional MR spectroscopy (MRS) suffers from low spatial resolution and relatively long acquisition times. Creatine chemical exchange saturation transfer (CrCEST) magnetic resonance imaging (MRI) is an emerging molecular imaging method for tissue Cr measurements. Our previous study showed that the CrCEST contrast, obtained through multicomponent Z-spectral fitting, was lower in tumors compared to normal brain, which further reduced with tumor progression. The current study was aimed to investigate if CrCEST MRI can also be useful for differentiating gliomas with different degrees of aggressiveness.Procedures: Intracranial 9L gliosarcoma and F98 glioma bearing rats with matched tumor size were scanned with a 9.4 T MRI scanner at two time points. CEST Z-spectra were collected using a customized sequence with a frequency-selective rectangular saturation pulse (B1 = 50 Hz, duration = 3 s) followed by a single-shot readout. Z spectral data were fitted pixel-wise with five Lorentzian functions, and maps of CrCEST peak amplitude, linewidth, and integral were produced. For comparison, single-voxel proton MR spectroscopy (1H-MRS) was performed to quantify and compare the total Cr concentration in the tumor.Results: CrCEST contrasts decreased with tumor progression from weeks 3 to 4 in both 9L and F98 phenotypes. More importantly, F98 tumors had significantly lower CrCEST integral compared to 9L tumors. On the other hand, integrals of other Z-spectral components were unable to differentiate both tumor progression and phenotype with limited sample size.Conclusions: Given that F98 is a more aggressive tumor than 9L, this study suggests that CrCEST MRI may help differentiate gliomas with different aggressiveness.
      pubtype: Academic Journal
      doctype:
        diagnostic images
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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