High-Throughput Approaches to the Development of Molecular Imaging Agents.
Molecular imaging allows for the visualization of changes at the cellular level in diseases such as cancer. A successful molecular imaging agent must rely on disease-selective targets and ligands that specifically interact with those targets. Unfortunately, the translation of novel target-specific l...
| Publicado en: | Molecular Imaging & Biology Vol. 19; no. 2; pp. 163 - 183 |
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| Autores principales: | , , , , , |
| Formato: | review Journal Article |
| Publicado: |
Springer Nature
Apr2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121548619&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121548619 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15361632 KJU jtl: Molecular Imaging & Biology issn: 15361632 maglogo: N pubinfo: dt: Apr2017 vid: 19 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 121548619 121548619 143902583 NLM27812924 121548619 10.1007/s11307-016-1016-z NLM27812924 121548619 ppf: 163 ppct: 20 formats: fmt: @attributes: type: P tig: atl: High-Throughput Approaches to the Development of Molecular Imaging Agents. aug: au: Hu, Lina Kelly, Kimberly Sutcliffe, Julie Hu, Lina Y Kelly, Kimberly A Sutcliffe, Julie L affil: Department of Biomedical Engineering , University of California Davis , Davis USA sug: subj: Molecular Imaging Methods High-Throughput Screening Assays Methods Amino Acids Peptides Animals Technology, Pharmaceutical Indicators and Reagents Scales ab: Molecular imaging allows for the visualization of changes at the cellular level in diseases such as cancer. A successful molecular imaging agent must rely on disease-selective targets and ligands that specifically interact with those targets. Unfortunately, the translation of novel target-specific ligands into the clinic has been frustratingly slow with limitations including the complex design and screening approaches for ligand identification, as well as their subsequent optimization into useful imaging agents. This review focuses on combinatorial library approaches towards addressing these two challenges, with particular focus on phage display and one-bead one-compound (OBOC) libraries. Both of these peptide-based techniques have proven successful in identifying new ligands for cancer-specific targets and some of the success stories will be highlighted. New developments in screening methodology and sequencing technology have pushed the bounds of phage display and OBOC even further, allowing for even faster and more robust discovery of novel ligands. The combination of multiple high-throughput technologies will not only allow for more accurate identification, but also faster affinity maturation, while overall streamlining the process of translating novel ligands into clinical imaging agents. pubtype: Academic Journal doctype: review Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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