Minimal residual disease monitoring in childhood B lymphoblastic leukemia with t(12;21)(p13;q22); ETV6- RUNX1: concordant results using quantitation of fusion transcript and flow cytometry.
Introduction The translocation t(12;21)(p13;q22) resulting in the fusion gene ETV6- RUNX1, is the most frequent gene fusion in childhood B lymphoblastic leukemia. In the Nordic Society of Paediatric Haematology and Oncology ALL-2008 treatment protocol, treatment stratification in B-lineage ALL is ba...
| Publicado en: | International Journal of Laboratory Hematology Vol. 39; no. 2; pp. 121 - 129 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Apr2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121807464&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121807464 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17515521 47EA jtl: International Journal of Laboratory Hematology issn: 17515521 maglogo: Y pubinfo: dt: Apr2017 vid: 39 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 121807464 121807464 121807464 10.1111/ijlh.12593 121807464 ppf: 121 ppct: 8 formats: tig: atl: Minimal residual disease monitoring in childhood B lymphoblastic leukemia with t(12;21)(p13;q22); ETV6- RUNX1: concordant results using quantitation of fusion transcript and flow cytometry. aug: au: Alm, S. J. Engvall, C. Asp, J. Palmqvist, L. Abrahamsson, J. Fogelstrand, L. affil: Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg Sweden sug: subj: Flow Cytometry Leukemia, Lymphocytic Diagnosis Disease Surveillance Bone Marrow Physiology Child Human Leukemia, Lymphocytic Physiopathology Genes Genetics Medical Organizations Pediatrics Hematology Cell Physiology Polymerase Chain Reaction Fluorescence Polarization Immunoassay Wilcoxon Rank Sum Test Data Analysis Software Male Female Funding Source Child: 6-12 years Male Female ab: Introduction The translocation t(12;21)(p13;q22) resulting in the fusion gene ETV6- RUNX1, is the most frequent gene fusion in childhood B lymphoblastic leukemia. In the Nordic Society of Paediatric Haematology and Oncology ALL-2008 treatment protocol, treatment stratification in B-lineage ALL is based on results of minimal residual disease ( MRD) analysis with fluorescence-activated cell sorting ( FACS). In this study, we determined whether RT- qPCR of the ETV6- RUNX1 fusion transcript can be a reliable alternative for MRD analysis. Methods Seventy-eight bone marrow samples from 29 children at diagnosis and day 15, 29, and 78 during treatment were analyzed for MRD with FACS and with quantitative reverse transcription polymerase chain reaction ( RT- qPCR). Fusion transcript MRD was defined as the ETV6- RUNX1/ GUSB ratio at the follow-up time point (day 15/29/78) divided with the ETV6- RUNX1/ GUSB ratio at diagnosis (%). Results MRD analysis with FACS and with RT- qPCR of ETV6- RUNX1 fusion transcript showed strong correlation. All cases showed concordant results at the treatment stratifying time points day 29 and day 78, when comparing the two methods with a cutoff set to 0.1%. Conclusion RT- qPCR is a valuable addition and could also be an alternative to FACS in cases where FACS is not achievable for MRD analysis. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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