Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise.
Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model. Methods. A rat model of CFS was established using restraint-stress, forced exercise, and...
| Publicado en: | BioMed Research International Vol. 2017; pp. 1 - 13 |
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| Autores principales: | , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
3/22/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=121994097&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 121994097 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 3/22/2017 vid: 2017 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 121994097 121994097 121994097 10.1155/2017/8182020 121994097 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Detection of Urine Metabolites in a Rat Model of Chronic Fatigue Syndrome before and after Exercise. aug: au: Shao, Changzhuan Ren, Yiming Wang, Zinan Kang, Chenzhe Jiang, Hongke Chi, Aiping affil: College of Arts and Sciences, Shanghai Maritime University, Shanghai 201306, China sug: subj: Body Fluids Fatigue Syndrome, Chronic Diagnosis Urinalysis Exercise Animal Studies Fatigue Syndrome, Chronic Physiopathology Rats Gas Chromatography-Mass Spectrometry Models, Biological Multivariate Analysis Data Analysis Software Metabolism Tricarboxylic Acids Alanine Metabolism Aspartate Aminotransferase Female Behavior Funding Source Female ab: Purpose. The aim of the present study was to elucidate the metabolic mechanisms associated with chronic fatigue syndrome (CFS) via an analysis of urine metabolites prior to and following exercise in a rat model. Methods. A rat model of CFS was established using restraint-stress, forced exercise, and crowded and noisy environments over a period of 4 weeks. Behavioral experiments were conducted in order to evaluate the model. Urine metabolites were analyzed via gas chromatography-mass spectrometry (GC-MS) in combination with multivariate statistical analysis before and after exercise. Results. A total of 20 metabolites were detected in CFS rats before and after exercise. Three metabolic pathways (TCA cycle; alanine, aspartate, and glutamate metabolism; steroid hormone biosynthesis) were significantly impacted before and after exercise, while sphingolipid metabolism alone exhibited significant alterations after exercise only. Conclusion. In addition to metabolic disturbances involving some energy substances, alterations in steroid hormone biosynthesis and sphingolipid metabolism were detected in CFS rats. Sphingosine and 21-hydroxypregnenolone may be key biomarkers of CFS, potentially offering evidence in support of immune dysfunction and hypothalamic-pituitary-adrenal (HPA) axis hypoactivity in patients with CFS. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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