Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo.
Background and Objective: Diagnosis of esophageal diseases is often hampered by sampling errors that are inherent in endoscopic biopsy, the standard of care. Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance confocal endomicroscopy technology that has the potential to visuali...
| Publicado en: | Lasers in Surgery & Medicine Vol. 49; no. 3; pp. 233 - 240 |
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| Autores principales: | , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Mar2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=122080390&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 122080390 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01968092 OCY jtl: Lasers in Surgery & Medicine issn: 01968092 maglogo: Y pubinfo: dt: Mar2017 vid: 49 iid: 3 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 122080390 122080390 NLM27636715 122080390 10.1002/lsm.22585 NLM27636715 122080390 ppf: 233 ppct: 7 formats: tig: atl: Large-area spectrally encoded confocal endomicroscopy of the human esophagus in vivo. aug: au: Kang, Dongkyun Schlachter, Simon C. Carruth, Robert W. Kim, Minkyu Wu, Tao Tabatabaei, Nima Soomro, Amna R. Grant, Catriona N. Rosenberg, Mireille Nishioka, Norman S. Tearney, Guillermo J. affil: Wellman Center for Photomedicine, Massachusetts General Hospital, Boston Massachusetts 02114 sug: subj: Microscopy Methods Precancerous Conditions Pathology Barrett Esophagus Pathology Esophageal Neoplasms Pathology Endoscopy, Digestive System Methods Biopsy, Needle Male Immunohistochemistry Pilot Studies Precancerous Conditions Diagnosis Esophageal Neoplasms Diagnosis Probability Sample Barrett Esophagus Diagnosis Diagnosis, Differential Female Monitoring, Physiologic Methods Human Male Female ab: Background and Objective: Diagnosis of esophageal diseases is often hampered by sampling errors that are inherent in endoscopic biopsy, the standard of care. Spectrally encoded confocal microscopy (SECM) is a high-speed reflectance confocal endomicroscopy technology that has the potential to visualize cellular features from large regions of the esophagus, greatly decreasing the likelihood of sampling error. In this paper, we report results from a pilot clinical study imaging the human esophagus in vivo with a prototype SECM endoscopic probe.Materials and Methods: In this pilot clinical study, six patients undergoing esophagogastroduodenoscopy (EGD) for surveillance of Barrett's esophagus (BE) were imaged with the SECM endoscopic probe. The device had a diameter of 7 mm, a length of 2 m, and a rapid-exchange guide wire provision for esophageal placement. During EGD, the distal portion of the esophagus of each patient was sprayed with 2.5% acetic acid to enhance nuclear contrast. The SECM endoscopic probe was then introduced over the guide wire to the distal esophagus and large-area confocal images were obtained by helically scanning the optics within the SECM probe.Results: Large area confocal images of the distal esophagus (image length = 4.3-10 cm; image width = 2.2 cm) were rapidly acquired at a rate of ∼9 mm2 /second, resulting in short procedural times (1.8-4 minutes). SECM enabled the visualization of clinically relevant architectural and cellular features of the proximal stomach and normal and diseased esophagus, including squamous cell nuclei, BE glands, and goblet cells.Conclusions: This study demonstrates that comprehensive spectrally encoded confocal endomicroscopy is feasible and can be used to visualize architectural and cellular microscopic features from large segments of the distal esophagus at the gastroesophageal junction. By providing microscopic images that are less subject to sampling error, this technology may find utility in guiding biopsy and planning and assessing endoscopic therapy. Lasers Surg. Med. 49:233-239, 2017. © 2016 Wiley Periodicals, Inc. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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