Isolated metaphyseal injury influences unrelated bones.
Background and purpose — Fracture healing involves different inflammatory cells, some of which are not part of the traditional bone field, such as B-cells and cytotoxic T-cells. We wanted to characterize bone healing by flow cytometry using 15 different inflammatory cell markers in a mouse model of...
| Publicado en: | Acta Orthopaedica Vol. 88; no. 2; pp. 223 - 231 |
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| Autores principales: | , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Medical Journals Sweden AB
Apr2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=122253574&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 122253574 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17453674 1BVL jtl: Acta Orthopaedica issn: 17453674 maglogo: N pubinfo: dt: Apr2017 vid: 88 iid: 2 pid: 59195 pub: Medical Journals Sweden AB artinfo: ui: 122253574 122253574 122253574 10.1080/17453674.2016.1274587 122253574 ppf: 223 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Isolated metaphyseal injury influences unrelated bones. aug: au: Tätting, Love Sandberg, Olof Bernhardsson, Magnus Ernerudh, Jan Aspenberg, Per affil: Department of Clinical and Experimental Medicine, Orthopaedics sug: subj: Fracture Healing Flow Cytometry Tibia Humerus Models, Biological Mice Inflammation Osteogenesis Leukocytes ab: Background and purpose — Fracture healing involves different inflammatory cells, some of which are not part of the traditional bone field, such as B-cells and cytotoxic T-cells. We wanted to characterize bone healing by flow cytometry using 15 different inflammatory cell markers in a mouse model of metaphyseal injury, and incidentally discovered a previously unknown general skeletal reaction to trauma. Material and methods — A bent needle was inserted and twisted to traumatize the cancellous bone in the proximal tibia of C57/Bl6 female mice. This is known to induce vivid bone formation locally in the marrow compartment. Cells were harvested from the injured region, the uninjured contralateral tibia, and the humerus. The compositions of the immune cell populations were compared to those in untraumatized control animals. Results — Tibial metaphyseal injury led to substantial changes in the cell populations over time. Unexpectedly, similar changes were also seen in the contralateral tibia and in the humerus, despite the lack of local trauma. Most leukocyte subsets were affected by this generalized reaction. Interpretation — A relatively small degree of injury to the proximal tibia led to systemic changes in the immune cell populations in the marrow of unrelated bones, and probably in the entire skeleton. The few changes that were specific for the injury site appeared to relate to modulatory functions. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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