Delivery of Parasite RNA Transcripts Into Infected Epithelial Cells During Cryptosporidium Infection and Its Potential Impact on Host Gene Transcription.
Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human i...
| Publicado en: | Journal of Infectious Diseases Vol. 215; no. 4; pp. 636 - 644 |
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| Autores principales: | , , , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
2/15/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=122320068&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 122320068 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00221899 1YU jtl: Journal of Infectious Diseases issn: 00221899 maglogo: N pubinfo: dt: 2/15/2017 vid: 215 iid: 4 pid: 622 pub: Oxford University Press / USA artinfo: ui: 122320068 122320068 NLM28007919 122320068 10.1093/infdis/jiw607 NLM28007919 122320068 ppf: 636 ppct: 8 formats: tig: atl: Delivery of Parasite RNA Transcripts Into Infected Epithelial Cells During Cryptosporidium Infection and Its Potential Impact on Host Gene Transcription. aug: au: Yang Wang Ai-Yu Gong Shibin Ma Xiqiang Chen Yan Li Chun-Jen Su Norall, Dana Jing Chen Strauss-Soukup, Juliane K. Xian-Ming Chen Wang, Yang Gong, Ai-Yu Ma, Shibin Chen, Xiqiang Li, Yan Su, Chun-Jen Chen, Jing Chen, Xian-Ming affil: Department of Medical Microbiology and Immunology, Creighton University School of Medicine sug: subj: Epithelial Cells RNA Cryptosporidiosis Epithelial Cells Metabolism Proteins Metabolism Cell Line Proteins Intestines Metabolism Cryptosporidium Intestines Gene Expression Profiling Funding Source Human ab: Cryptosporidium parvum is an important opportunistic parasite pathogen for immunocompromised individuals and a common cause of diarrhea in young children. Previous studies have identified a panel of RNA transcripts of very low protein-coding potential in C. parvum. Using an in vitro model of human intestinal cryptosporidiosis, we report here that some of these C. parvum RNA transcripts were selectively delivered into the nuclei of host epithelial cells during C. parvum infection. Nuclear delivery of several such parasitic RNAs, including Cdg7_FLc_0990, involved heat-shock protein 70-mediated nuclear importing mechanism. Overexpression of Cdg7_FLc_0990 in intestinal epithelial cells resulted in significant changes in expression levels of specific genes, with significant overlapping with alterations in gene expression profile detected in host cells after C. parvum infection. Our data demonstrate that C. parvum transcripts of low protein-coding potential are selectively delivered into epithelial cells during infection and may modulate gene transcription in infected host cells. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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