Quantified coronary frequency domain optical coherence tomography signal analysis for the evaluation of erythrocyte-rich thrombus: ex-vivo validation study.
Previous study has demonstrated that erythrocyte-rich thrombi contain more inflammatory cells and reflect high thrombus burden, leading to impaired myocardial reperfusion in myocardial infarction. The aim of this study is to investigate the utility of quantified frequency domain optical coherence to...
| Publicado en: | International Journal of Cardiovascular Imaging Vol. 33; no. 5; pp. 587 - 595 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
May2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=122340584&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 122340584 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15695794 1HHY jtl: International Journal of Cardiovascular Imaging issn: 15695794 maglogo: N pubinfo: dt: May2017 vid: 33 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 122340584 122340584 144134503 NLM28025722 122340584 10.1007/s10554-016-1038-2 NLM28025722 122340584 ppf: 587 ppct: 8 formats: tig: atl: Quantified coronary frequency domain optical coherence tomography signal analysis for the evaluation of erythrocyte-rich thrombus: ex-vivo validation study. aug: au: Shiratori, Yoshitaka Watanabe, Masato Suzuki, Nobuaki Fukusato, Toshio Kozuma, Ken affil: Department of Internal Medicine , Teikyo University School of Medicine , 2-11-1 Kaga Itabashi-ku Tokyo 173-8605 Japan sug: subj: Erythrocytes Pathology Coronary Thrombosis Tomography, Optical Coherence Coronary Arteriosclerosis Pharmacokinetics Thrombectomy Aged, 80 and Over Male Coronary Thrombosis Blood Image Interpretation, Computer Assisted Coronary Thrombosis Therapy Aged Coronary Arteriosclerosis Blood Middle Age Software Reproducibility of Results Predictive Value of Tests Female Coronary Arteriosclerosis Therapy Coronary Thrombosis Pathology Biopsy Coronary Arteriosclerosis Pathology ROC Curve Cardiovascular Care Human Aged, 80 & over Aged: 65+ years Middle Aged: 45-64 years Male Female ab: Previous study has demonstrated that erythrocyte-rich thrombi contain more inflammatory cells and reflect high thrombus burden, leading to impaired myocardial reperfusion in myocardial infarction. The aim of this study is to investigate the utility of quantified frequency domain optical coherence tomography (FD-OCT) signal analysis in evaluating the erythrocyte-rich thrombus with ex-vivo materials. We evaluated 54 specimens of coronary artery thrombus obtained by thrombectomy catheter from 8 patients who underwent primary percutaneous coronary intervention. The thrombi were immersed in saline immediately after thrombectomy and FD-OCT image acquisition was performed ex-vivo. Quantitative analysis for all contiguous frames was performed by the dedicated automated software (OCT system software, Light Lab Inc.). For the maximum thrombus area, mean signal intensity (MSI) and normalized standard deviation of signal (NSD) was evaluated. All thrombi were stained using double staining of phosphotungstic acid-hematoxylin and eosin to enable automatic extraction of erythrocyte from fibrin. Computer-assisted analysis was performed using dedicated image processing software (WinROOF, Mitani Corp., Tokyo, Japan) for color identification of the erythrocyte area. Erythrocyte-rich thrombus, defined as % erythrocyte [(erythrocyte area/total thrombus area) × 100] ≥ 10%, showed significantly lower MSI [4.39 ± 0.24 vs. 4.74 ± 0.35, p = 0.002] than that of <10%. The cut-off point for prediction of erythrocyte-rich thrombus was defined as MSI ≤ 4.56, sensitivity: 87.5%, specificity: 82.9%, area under the curve: 0.836, respectively). The present ex-vivo study suggested the utility of quantified FD-OCT signal analysis on the detection of erythrocyte-rich thrombus. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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