Expression of YAP/TAZ in Keratocystic Odontogenic Tumors and Its Possible Association with Proliferative Behavior.

The aim of this study is to clarify whether YAP/TAZ is involved in the pathogenesis and proliferative growth of keratocystic odontogenic tumor (KCOT). The expression levels of YAP/TAZ and downstream proteins and genes in normal oral mucosa (OM) and KCOT were determined and compared by immunohistoche...

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Publicado en:BioMed Research International Vol. 2017; pp. 1 - 8
Autores principales: Man, Qi-Wen, Ma, Yan-Qi, Liu, Jin-Yuan, Zhao, Yi, Liu, Bing, Zhao, Yi-Fang
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 4/23/2017
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 4/23/2017
      vid: 2017
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        122637390
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        10.1155/2017/4624890
        122637390
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        atl: Expression of YAP/TAZ in Keratocystic Odontogenic Tumors and Its Possible Association with Proliferative Behavior.
      aug:
        au:
          Man, Qi-Wen
          Ma, Yan-Qi
          Liu, Jin-Yuan
          Zhao, Yi
          Liu, Bing
          Zhao, Yi-Fang
        affil: The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China
      sug:
        subj:
          Odontogenic Tumors Physiopathology
          Cell Proliferation
          Gene Expression
          Transcription Factors
          Immunohistochemistry
          Polymerase Chain Reaction
          Genetics
          Descriptive Statistics
          Spearman's Rank Correlation Coefficient
          Mouth Mucosa Analysis
          Genes
          Human
      ab: The aim of this study is to clarify whether YAP/TAZ is involved in the pathogenesis and proliferative growth of keratocystic odontogenic tumor (KCOT). The expression levels of YAP/TAZ and downstream proteins and genes in normal oral mucosa (OM) and KCOT were determined and compared by immunohistochemistry and real-time quantitative PCR. The results showed that the expression of YAP/TAZ and downstream proteins (Cyr61, CTGF) was significantly upregulated in KCOT with upregulation of Ki-67 compared to OM. Importantly, the mRNA levels of transcription factors (TEAD1, TEAD4, and RUNX2) and cell cycle related genes (CDK2, PCNA), which interact with the transcriptional coactivators YAP/TAZ, are also upregulated in the KCOT. In addition, the results from Spearman rank correlation test revealed the close relationship between YAP/TAZ and Ki-67, which was further evidenced by double-labelling immunofluorescence that revealed a synchronous distribution for YAP/TAZ with Ki-67 in KCOT samples. All the data suggested YAP/TAZ might be involved in the proliferative behavior of KCOT.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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