Involvement of p53 and Bcl-2 in sensory cell degeneration in aging rat cochleae.
Conclusion: p53 and Bcl-2 (B-cell lymphoma 2) are involved in the process of sensory cell degeneration in aging cochleae. Objective: To determine molecular players in age-related hair cell degeneration, this study examined the changes in p53 and Bcl-2 expression at different stages of apoptotic and...
| Publicado en: | Acta Oto-Laryngologica Vol. 137; no. 6; pp. 572 - 581 |
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| Autores principales: | , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Jun2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=122691960&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 122691960 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00016489 B6D jtl: Acta Oto-Laryngologica issn: 00016489 maglogo: Y pubinfo: dt: Jun2017 vid: 137 iid: 6 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 122691960 122691960 122691960 10.1080/00016489.2016.1274425 122691960 ppf: 572 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Involvement of p53 and Bcl-2 in sensory cell degeneration in aging rat cochleae. aug: au: Xu, Yang Yang, Wei Ping Hu, Bo Hua Yang, Shiming Henderson, Donald affil: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, PR China sug: subj: Cochlea Aging Intracellular Signaling Peptides and Proteins Analysis Hair Cells Animal Studies Rats Apoptosis Necrosis Evoked Potentials, Auditory, Brainstem Epithelium Staining and Labeling Cell Culture Techniques P-Value Descriptive Statistics ab: Conclusion: p53 and Bcl-2 (B-cell lymphoma 2) are involved in the process of sensory cell degeneration in aging cochleae. Objective: To determine molecular players in age-related hair cell degeneration, this study examined the changes in p53 and Bcl-2 expression at different stages of apoptotic and necrotic death of hair cells in aging rat cochleae. Methods: Young (3–4 months) and aging (23–24 months) Fisher 344/NHsd rats were used. The thresholds of the auditory brainstem response (ABR) were measured to determine the auditory function. Immunolabeling was performed to determine the expression of p53 and Bcl-2 proteins in the sensory epithelium. Propidium iodide staining was performed to determine the morphologic changes in hair cell nuclei. Results: Aging rats exhibited a significant elevation in ABR thresholds at all tested frequencies (p < 0.001). The p53 and Bcl-2 immunoreactivity was increased in aging hair cells showing the early signs of apoptotic changes in their nuclei. The Bcl-2 expression increase was also observed in hair cells displaying early signs of necrosis. As the hair cell degenerative process advanced, p53 and Bcl-2 immunoreactivity became reduced or absent. In the areas where no detectable nuclear staining was present, p53 and Bcl-2 immunoreactivity was absent. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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