Multicolor immunofluorescence reveals that p63- and/or K5-positive progenitor cells contribute to normal breast epithelium and usual ductal hyperplasia but not to low-grade intraepithelial neoplasia of the breast.
We contend that knowledge about the cellular composition of normal breast epithelium is a prerequisite for understanding proliferative breast disease. Against this background, we used multicolor immunofluorescence to study normal breast epithelium and two types of intraepithelial proliferative breas...
| Publicado en: | Virchows Archiv: European Journal of Pathology Vol. 470; no. 5; pp. 493 - 505 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
May2017
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=122710508&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 122710508 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09456317 O1Z jtl: Virchows Archiv: European Journal of Pathology issn: 09456317 maglogo: N pubinfo: dt: May2017 vid: 470 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 122710508 122710508 144085047 NLM28303349 122710508 10.1007/s00428-017-2073-7 NLM28303349 122710508 ppf: 493 ppct: 12 formats: fmt: @attributes: type: P tig: atl: Multicolor immunofluorescence reveals that p63- and/or K5-positive progenitor cells contribute to normal breast epithelium and usual ductal hyperplasia but not to low-grade intraepithelial neoplasia of the breast. aug: au: Boecker, Werner Stenman, Göran Schroeder, Tina Schumacher, Udo Loening, Thomas Stahnke, Lisa Löhnert, Catharina Siering, Robert Kuper, Arthur Samoilova, Vera Tiemann, Markus Korsching, Eberhard Buchwalow, Igor Stenman, Göran Löhnert, Catharina Siering, Robert Michael affil: Sahlgrenska Cancer Center , University of Gothenburg , 405 30 Gothenburg Sweden sug: subj: Stem Cells Breast Neoplasms Pathology Breast Precancerous Conditions Pathology Carcinoma in Situ Pathology Image Processing, Computer Assisted Membrane Proteins Aged Middle Age Antigens, Bacterial Hyperplasia Pathology Adult Female Fluorescent Antibody Technique Antigens, Surface Human Aged: 65+ years Middle Aged: 45-64 years Adult: 19-44 years Female ab: We contend that knowledge about the cellular composition of normal breast epithelium is a prerequisite for understanding proliferative breast disease. Against this background, we used multicolor immunofluorescence to study normal breast epithelium and two types of intraepithelial proliferative breast lesion for expression of the p63, basal keratin K5, glandular keratin K8/18, SMA, ER-alpha, and Ki67. We studied eight normal breast epithelium samples, 12 cases of usual ductal hyperplasia, and 33 cases of low-grade intraepithelial neoplasia (9 flat epithelial atypia, 14 low-grade ductal carcinoma in situ and 10 cases of lobular neoplasia). Usual ductal hyperplasia showed striking similarity to normal luminal breast epithelium including p63+ and/or K5+ luminal progenitor cells and the full spectrum of luminal progeny cells. In normal breast epithelium and usual ductal hyperplasia, expression of ER-alpha was associated with lack of expression of the proliferation antigen Ki67. In contrast, we found in both types of low-grade intraepithelial neoplasia robust expression of keratin K8/18 and a positive association between ER-alpha and Ki67 expression. However, these lesions were consistently negative for p63 and/or K5. Our observational study supports the view that usual ductal hyperplasia and low-grade intraepithelial neoplasia are different entities rather than part of a spectrum of the same disease. We propose a new operational model of cell differentiation that may serve to better understand correlations between normal breast epithelium and proliferative breast diseases. From our data we conclude that p63+ and/or K5+ progenitor cells contribute to maintenance of normal epithelium and usual ductal hyperplasia, but not to low-grade intraepithelial neoplasia of the breast. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|