TIMELESS contributes to the progression of breast cancer through activation of MYC.
Background: Breast cancer is the most common malignancy and the leading cause of cancer death among women. TIMELESS (TIM), a circadian rhythm regulator, has been recently implicated in the progression of human cancer. However, the role of TIM in the progression of breast cancer has not been well-cha...
| Publicado en: | Breast Cancer Research Vol. 19; pp. 1 - 11 |
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| Autores principales: | , , , , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
BioMed Central
5/2/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=123053465&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 123053465 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14655411 8UYJ jtl: Breast Cancer Research issn: 14655411 maglogo: N pubinfo: dt: 5/2/2017 vid: 19 pid: 24147 pub: BioMed Central artinfo: ui: 123053465 123053465 NLM28464854 123053465 10.1186/s13058-017-0838-1 NLM28464854 123053465 ppf: 1 ppct: 10 formats: fmt: @attributes: type: P tig: atl: TIMELESS contributes to the progression of breast cancer through activation of MYC. aug: au: Limin Chi Yujiao Zou Ling Qin Weifeng Ma Yanyan Hao Yao Tang Rongcheng Luo Ziqing Wu Chi, Limin Zou, Yujiao Qin, Ling Ma, Weifeng Hao, Yanyan Tang, Yao Luo, Rongcheng Wu, Ziqing affil: Cancer Center, TCM-Integrated Hospital, Southern Medical University, Guangzhou 510315, China sug: subj: Proteins Intracellular Signaling Peptides and Proteins Breast Neoplasms Cell Physiology Cell Cycle Proteins Disease Progression Cell Line, Tumor Female Neoplasm Invasiveness Breast Neoplasms Pathology Genes Stem Cells Pathology Human Female ab: Background: Breast cancer is the most common malignancy and the leading cause of cancer death among women. TIMELESS (TIM), a circadian rhythm regulator, has been recently implicated in the progression of human cancer. However, the role of TIM in the progression of breast cancer has not been well-characterized.Methods: Immunohistochemistry (IHC) staining was used to examine TIM levels in breast cancer specimens. Mammosphere formation analysis and side population analysis were used to examine the effect of TIM on the self-renewal of breast cancer stem cells. A wound healing assay and a Transwell assay were used to determine the role of TIM in breast cancer cell migration and invasion. A soft agar growth assay in vitro and tumorigenicity in vivo were used to determine the role of TIM in tumorigenicity.Results: TIM levels in both breast cancer cell lines and tissues were significantly upregulated. Patients with high TIM had poorer prognosis than patients with low TIM. Overexpression of TIM dramatically enhanced, while knockdown of TIM suppressed the self-renewal of cancer stem cells (CSCs), cell invasion and migration abilities of breast cancer cells in vitro. Moreover, overexpression of TIM significantly augmented, while knockdown of TIM reduced the tumorigenicity of breast cancer cells in vivo. Mechanism studies revealed that TIM upregulated the expression and the trans-activity of the well-known oncogene MYC. Inhibition of MYC significantly blocked the effects of TIM on CSC population, cell invasion and anchor-independent cell growth.Conclusion: TIM plays an important role in promoting breast cancer progression and may represent a novel therapeutic target for breast cancer. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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