Rethinking Autoantibody Signature Panels for Cancer Diagnosis.

Introduction: Most pulmonary nodules found on imaging studies are indeterminate, but because of the concern for lung cancer, all patients require further evaluation with resultant radiation risk, significant cost, and delays in diagnosis. We hypothesized that a diagnostic blood test based on detecti...

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Publicado en:Journal of Thoracic Oncology Vol. 12; no. 6; pp. 1011 - 1015
Autores principales: Campa, Michael J., Gottlin, Elizabeth B., IIHerndon, James E., Jr.Patz, Edward F., Herndon, James E 2nd, Patz, Edward F Jr
Formato: research Journal Article
Publicado: Elsevier B.V. Jun2017
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2017
      vid: 12
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      pub: Elsevier B.V.
      place: New York, New York
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        10.1016/j.jtho.2017.01.017
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        atl: Rethinking Autoantibody Signature Panels for Cancer Diagnosis.
      aug:
        au:
          Campa, Michael J.
          Gottlin, Elizabeth B.
          IIHerndon, James E.
          Jr.Patz, Edward F.
          Herndon, James E 2nd
          Patz, Edward F Jr
        affil: Department of Radiology, Duke University Medical Center, Durham, North Carolina
      sug:
        subj:
          Carcinoma, Non-Small-Cell Lung Diagnosis
          Autoantibodies Blood
          Lung Neoplasms Diagnosis
          Antigens, Tumor Immunology
          Lung Neoplasms Immunology
          Prognosis
          Lung Neoplasms Blood
          Carcinoma, Non-Small-Cell Lung Blood
          Algorithms
          Protein Array Analysis
          Carcinoma, Non-Small-Cell Lung Immunology
          Human
      ab: Introduction: Most pulmonary nodules found on imaging studies are indeterminate, but because of the concern for lung cancer, all patients require further evaluation with resultant radiation risk, significant cost, and delays in diagnosis. We hypothesized that a diagnostic blood test based on detection of autoantibodies against cancer antigens would be able to distinguish a benign nodule from lung cancer.Methods: We identified a panel of 25 serum autoantibodies associated with NSCLC and constructed a protein microarray containing the autoantigens. We tested the microarray with human sera (from 125 patients with NSCLC and 125 matched controls with a benign nodule) and attempted to develop a classification algorithm that would separate the two groups.Results: In the training data set the logistic regression c-index statistic was 0.691; in the validation data set, the model predicting the score generated from the training set model had a c-index of 0.490. The relationship between the score and outcome (final diagnosis) was not statistically significant (p = 0.460).Conclusions: When the current panel of antigens and assay format was used, classification algorithms based on levels of autoantibodies to cancer antigens did not prove to have statistically significant value for predicting the presence of cancer. We suggest that there are inherent biological limitations to this approach.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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