GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.
Glycyl- l-histidyl- l-lysine (GHK)-Cu is considered to be an activator of tissue remodeling, and has been used in cosmetic products. In this study, we prepared liposomes encapsulating GHK-Cu and analyzed their effect on human umbilical vein endothelial cells (HUVECs) proliferation and scald wound he...
| Published in: | Wound Repair & Regeneration Vol. 25; no. 2; pp. 270 - 279 |
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| Main Authors: | , , , , , , , , , |
| Format: | pictorial research tables/charts tracings Journal Article |
| Published: |
Wiley-Blackwell
Apr/May2017
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=123588785&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 123588785 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Apr/May2017 vid: 25 iid: 2 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 123588785 123588785 123588785 10.1111/wrr.12520 123588785 ppf: 270 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. aug: au: Wang, Xinying Liu, Baoquan Xu, Qiang Sun, Haiyang Shi, Meijun Wang, Dan Guo, Meihua Yu, Jiawen Zhao, Chunhui Feng, Bin affil: Department of Biotechnology, Dalian Medical University, Dalian China sug: subj: Burns Therapy Wound Healing Cell Proliferation Neovascularization, Physiologic Peptides Physiology Animal Studies Models, Biological Mice Flow Cytometry Blotting, Western Vascular Endothelial Growth Factors Fibroblasts Fluorescent Antibody Technique ab: Glycyl- l-histidyl- l-lysine (GHK)-Cu is considered to be an activator of tissue remodeling, and has been used in cosmetic products. In this study, we prepared liposomes encapsulating GHK-Cu and analyzed their effect on human umbilical vein endothelial cells (HUVECs) proliferation and scald wound healing in mice. The nanoscaled GHK-Cu-liposomes promoted HUVECs proliferation, with a 33.1% increased rate. Flow cytometry analysis showed increased cell number at G1 stage and decreased cell number at G2 stage after GHK-Cu-liposomes treatment. Western blotting indicated that the expression of vascular endothelial growth factor and fibroblast grow factors-2 were both enhanced, as well as cell cycle-related proteins CDK4 and CyclinD1. In a mice scald model, angiogenesis in burned skin treated with GHK-Cu-liposomes was better compared with free GHK-Cu, and immunofluorescence analysis showed enhanced signal of CD31 and Ki67 in GHK-Cu-liposomes treated mice. Moreover, the wound healing time was shortened to 14 days post injury. Our results provide the evidence that GHK-Cu-liposomes could be utilized as a treatment for skin wounds. pubtype: Academic Journal doctype: pictorial research tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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