Molecular characterisation of a DREB gene from Sophora moorcroftiana, an endemic species of plateau.
Various plant species in the Qinghai-Tibet Plateau exposed to harsh conditions, such as low oxygen, drought, extremely low temperatures and salinity, have evolved both molecular and physiological adaptation strategies to deal with these multiple stresses. Sophora moorcroftiana (Benth.) Baker (Fabace...
| Publicado en: | Protoplasma Vol. 254; no. 4; pp. 1735 - 1742 |
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| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jul2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=123670287&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 123670287 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Jul2017 vid: 254 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 123670287 144010669 10.1007/s00709-016-1065-9 123670287 ppf: 1735 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Molecular characterisation of a DREB gene from Sophora moorcroftiana, an endemic species of plateau. aug: au: Li, Huie Zhang, Yanfu Guo, Qiqiang Yao, Weijie affil: Agricultural and Animal Husbandry College , Tibet University , Nyingchi 860000 People's Republic of China sug: ab: Various plant species in the Qinghai-Tibet Plateau exposed to harsh conditions, such as low oxygen, drought, extremely low temperatures and salinity, have evolved both molecular and physiological adaptation strategies to deal with these multiple stresses. Sophora moorcroftiana (Benth.) Baker (Fabaceae) is a highly drought-resistant endemic Sophora shrub species in the Qinghai-Tibet Plateau. In our previous study, a drought-induced DREB transcription factor gene was identified and was designated as SmDREB1. SmDREB1- GFP fusion construct was introduced into Arabidopsis protoplast to characterise the function of SmDREB1 in drought resistance. The results showed that SmDREB1 targets the nucleus of Arabidopsis protoplast. Ectopic expression of SmDREB1 in model plant species Arabidopsis was performed. The transgenic lines showed increasing expressions of drought marker genes including AtDHN, AtLEA, AtPIP2 ;2, AtPIP2;3 and AtRD29, increasing activities of antioxidant enzymes and proline contents and increasing light-use efficiency under drought stress as compared with the wild-type plants; SmDREB1 transgenic lines are more resistant to drought than wild-type plants. Therefore, the SmDREB1 is a drought-resistant transcription factor gene of S. moorcroftiana and could be a candidate in genetic engineering to improve drought resistance of plateau plant species. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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