ERF73/HRE1 is involved in HO production via hypoxia-inducible Rboh gene expression in hypoxia signaling.
Hypoxia deprives cells of energy and induces severe physical damage in embryophytes. Under hypoxia, the equilibrium between ethylene and HO affects the response of the transcription factor AtERF73/HRE1. To evaluate the role of AtERF73/HRE1 during hypoxia signaling, we used three independent AtERF73/...
| Publicado en: | Protoplasma Vol. 254; no. 4; pp. 1705 - 1715 |
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| Autores principales: | , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Jul2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=123670288&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 123670288 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Jul2017 vid: 254 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 123670288 143905891 10.1007/s00709-016-1064-x 123670288 ppf: 1705 ppct: 10 formats: fmt: @attributes: type: P tig: atl: ERF73/HRE1 is involved in HO production via hypoxia-inducible Rboh gene expression in hypoxia signaling. aug: au: Yang, Chin-Ying Huang, Yi-Chun Ou, Shang-Ling affil: Department of Agronomy , National Chung Hsing University , Taichung 40227 Taiwan sug: ab: Hypoxia deprives cells of energy and induces severe physical damage in embryophytes. Under hypoxia, the equilibrium between ethylene and HO affects the response of the transcription factor AtERF73/HRE1. To evaluate the role of AtERF73/HRE1 during hypoxia signaling, we used three independent AtERF73/HRE1 knockout lines to detect HO accumulation. The results revealed that under hypoxia, HO accumulation in the AtERF73/HRE1 knockout lines decreased, indicating that AtERF73/HRE1 uses a negative feedback regulation mechanism to influence the production of HO induced through hypoxia signal transduction. Quantitative RT-PCR analyses showed that oxygen deficiency had different effects on the expression of the hypoxia-induced genes Rboh B, D, G, and I in the AtERF73/HRE1 knockout lines. In particular, Rboh B and D expression were increased, whereas Rboh G expression was decreased. The expression of Rboh I was increased at 1 h but decreased at 3 h during hypoxia treatment in the AtERF73/HRE1 knockout lines. Similarly, the transcript levels of antioxidant and hypoxia-induced/ethylene response genes in the AtERF73/HRE1 knockout lines were affected by hypoxic stress, indicating that AtERF73/HRE1 is essential to hypoxia signal transduction in embryophytes. Additionally, in histochemical analysis, AtERF73/HRE1 promoter-induced GUS expression was detected in various plant parts throughout the plant growth process (e.g., leaves, inflorescences, siliques), particularly in the edges of mature leaves and guard cells. Taken together, our results confirm that AtERF73/HRE1 plays a role in HO production by affecting the hypoxia-induced expression of Rboh genes in hypoxia signal transduction. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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