Genetic Code Expansion in Zebrafish Embryos and Its Application to Optical Control of Cell Signaling.
Site-specific incorporation of unnatural amino acids into proteins provides a powerful tool to study protein function. Here we report genetic code expansion in zebrafish embryos and its application to the optogenetic control of cell signaling. We genetically encoded four unnatural amino acids with a...
| Publicado en: | Journal of the American Chemical Society Vol. 139; no. 27; pp. 9100 - 9104 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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American Chemical Society
7/12/2017
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=124087166&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 124087166 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 7/12/2017 vid: 139 iid: 27 pid: 997 pub: American Chemical Society artinfo: ui: 124087166 10.1021/jacs.7b02145 ppf: 9100 ppct: 4 formats: tig: atl: Genetic Code Expansion in Zebrafish Embryos and Its Application to Optical Control of Cell Signaling. aug: au: Liu, Jihe Hemphill, James Samanta, Subhas Tsang, Michael Deiters, Alexander affil: Department of Chemistry, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States Department of Developmental Biology, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, United States su: Zebra danio embryos Cellular signal transduction Genetic code Fish embryology Fish genetics Optogenetics Fishes sug: subj: Zebra danio embryos Cellular signal transduction Genetic code Fish embryology Fish genetics Optogenetics Fishes ab: Site-specific incorporation of unnatural amino acids into proteins provides a powerful tool to study protein function. Here we report genetic code expansion in zebrafish embryos and its application to the optogenetic control of cell signaling. We genetically encoded four unnatural amino acids with a diverse set of functional groups, which included a photocaged lysine that was applied to the light-activation of luciferase and kinase activity. This approach enables versatile manipulation of protein function in live zebrafish embryos, a transparent and commonly used model organism to study embryonic development. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2017 holdings: @attributes: islocal: N |
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