Shear-Induced Amyloid Formation in the Brain: II. An Experimental System for Monitoring Amyloid Shear Processes and Investigating Potential Spinal Tap Problems.
Liquid sheared amyloid-β (Aβ) initiates amyloid cascade reactions, producing unstable, potentially toxic oligomers. There is a need for new analytical tools with which to study these oligomers. A very small bore capillary flow system is proposed as a tool for studying the effects of liquid shear in...
| Publicado en: | Journal of Alzheimer's Disease Vol. 59; no. 2; pp. 543 - 558 |
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| Autor principal: | |
| Formato: | tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=124284785&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 124284785 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872877 FLR jtl: Journal of Alzheimer's Disease issn: 13872877 maglogo: N pubinfo: dt: 2017 vid: 59 iid: 2 pid: 20732 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 124284785 124284785 NLM28671126 124284785 10.3233/JAD-170259 NLM28671126 124284785 ppf: 543 ppct: 15 formats: tig: atl: Shear-Induced Amyloid Formation in the Brain: II. An Experimental System for Monitoring Amyloid Shear Processes and Investigating Potential Spinal Tap Problems. aug: au: Trumbore, Conrad N. affil: Department of Chemistry and Biochemistry, University of Delaware, Kennett Square, PA, USA. sug: subj: Peptides Metabolism Brain Metabolism Proteins Proteins Metabolism Chromatography, High Pressure Liquid Animals Peptides Alzheimer's Disease Metabolism Alzheimer's Disease Pathology Models, Biological ab: Liquid sheared amyloid-β (Aβ) initiates amyloid cascade reactions, producing unstable, potentially toxic oligomers. There is a need for new analytical tools with which to study these oligomers. A very small bore capillary flow system is proposed as a tool for studying the effects of liquid shear in amyloid research. This simple system consists of injecting a short cylindrical liquid sample plug containing dissolved amyloid into a liquid mobile phase flowing through an empty, very small internal diameter capillary tube. For liquid samples containing a single protein sample, under conditions in which there is laminar flow and limited sample protein molecular diffusion, chromatograms monitoring the optical protein absorbance of capillary effluent contain either one or two peaks, depending on the mobile phase flow rate. By controlling the sample diffusion times through changes in flow rate and/or capillary diameter, this tool can be used to generate aliquot samples with precise, reproducible amounts of shear for exploring the effects of variable shear on amyloid systems. The tool can be used for producing in-capillary stopped flow spectra of shear-stressed Aβ monomers as well as for kinetic studies of Aβ dimer- and oligomer-forming reactions between shear stressed Aβ monomers. Many other experiments are suggested using this experimental tool for studying the effects of shear on different Aβ and other amyloid systems, including testing for potentially serious amyloid sampling errors in spinal tap quantitative analysis. The technique has potential as both a laboratory research and a clinical tool. pubtype: Academic Journal doctype: tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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