Stabilization of circulating thyroglobulin mRNA transcripts in patients treated for differentiated thyroid carcinoma.
Background The clinical utility of serum thyroglobulin in the follow-up of patients with differentiated thyroid carcinoma may be compromised by the presence of endogenous antithyroglobulin antibodies. To prevent interference by antithyroglobulin antibodies several groups have developed real-time PCR...
| Publicado en: | Annals of Clinical Biochemistry Vol. 54; no. 5; pp. 558 - 567 |
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| Autores principales: | , , |
| Formato: | research Journal Article |
| Publicado: |
Sage Publications Inc.
Sep2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=124582684&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 124582684 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00045632 8NS jtl: Annals of Clinical Biochemistry issn: 00045632 maglogo: Y pubinfo: dt: Sep2017 vid: 54 iid: 5 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 124582684 124582684 NLM27629910 124582684 10.1177/0004563216671538 NLM27629910 124582684 ppf: 558 ppct: 9 formats: tig: atl: Stabilization of circulating thyroglobulin mRNA transcripts in patients treated for differentiated thyroid carcinoma. aug: au: Feddersen, Søren Bastholt, Lars Pedersen, Susanne M. affil: Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark sug: subj: Thyroid Neoplasms Blood Thyroid Neoplasms Blood Chemical Analysis Methods Globulins RNA Thyroid Neoplasms Therapy Genes RNA Blood Ferrans and Powers Quality of Life Index Human ab: Background The clinical utility of serum thyroglobulin in the follow-up of patients with differentiated thyroid carcinoma may be compromised by the presence of endogenous antithyroglobulin antibodies. To prevent interference by antithyroglobulin antibodies several groups have developed real-time PCR-based assays for quantification of blood thyroglobulin mRNA levels. For accurate quantification of thyroglobulin mRNA in blood preanalytical factors must be recognized and controlled. In this study, we evaluate the effect of different blood RNA stabilizing systems - the Tempus Blood RNA system and the PAXgene Blood RNA system - and storage time on RNA yield and quality, and thyroglobulin mRNA stability. Methods Blood samples from 11 patients previously treated for differentiated thyroid carcinoma were collected in K2-EDTA, Tempus and PAXgene tubes and maintained at room temperature. RNA was isolated following storage for 0 and 72 h, and RNA yield, integrity and purity was determined. Thyroglobulin, GAPDH and ACTB mRNA levels were quantified by semi-quantitative real-time PCR. Results The RNA yield was significantly higher for blood collected in Tempus tubes compared with PAXgene tubes following storage for 72 h at room temperature ( P = 0.0011). High-quality RNA could be extracted from blood collected in PAXgene and Tempus tubes. Blood collected in K2-EDTA tubes, but not in PAXgene and Tempus tubes, showed significant changes in thyroglobulin mRNA levels following storage for 72 h at room temperature ( P = 0.0263). Conclusions Stabilization of blood in PAXgene and Tempus tubes enables storage at room temperature for up to 72 h, without compromising thyroglobulin mRNA levels. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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