Formation of Fluorohydroxyapatite with Silver Diamine Fluoride.
Silver diamine fluoride (SDF) is found to promote remineralization and harden the carious lesion. Hydroxyapatite crystallization is a crucial process in remineralization; however, the role of SDF in crystal formation is unknown. We designed an in vitro experiment with calcium phosphate with differen...
| Publicado en: | Journal of Dental Research Vol. 96; no. 10; pp. 122 - 129 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Sage Publications Inc.
Sep2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=124755318&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 124755318 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00220345 1H7 jtl: Journal of Dental Research issn: 00220345 maglogo: Y pubinfo: dt: Sep2017 vid: 96 iid: 10 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 124755318 124755318 NLM28521107 10.1177/0022034517709738 NLM28521107 124755318 ppf: 122 ppct: 7 formats: tig: atl: Formation of Fluorohydroxyapatite with Silver Diamine Fluoride. aug: au: Mei, M. L. Nudelman, F. Marzec, B. Walker, J. M. Lo, E. C. M. Walls, A. W. Chu, C. H. affil: Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China sug: subj: Cariostatic Agents Quaternary Ammonium Compounds Minerals Tooth Remineralization Spectrum Analysis, Raman Microscopy, Electron X-Rays Crystallization In Vitro Studies Phosphates ab: Silver diamine fluoride (SDF) is found to promote remineralization and harden the carious lesion. Hydroxyapatite crystallization is a crucial process in remineralization; however, the role of SDF in crystal formation is unknown. We designed an in vitro experiment with calcium phosphate with different SDF concentrations (0.38, 1.52, 2.66, 3.80 mg/mL) to investigate the effect of this additive on the nucleation and growth of apatite crystals. Two control groups were also prepared-calcium phosphate (CaCl2·2H2O + K2HPO4 in buffer solution) and SDF (Ag[NH3]2F in buffer solution). After incubation at 37 oC for 24 h, the shape and organization of the crystals were examined by bright-field transmission electron microscopy and electron diffraction. Unit cell parameters of the obtained crystals were determined with powder X-ray diffraction. The vibrational and rotational modes of phosphate groups were analyzed with Raman microscopy. The transmission electron microscopy and selected-area electron diffraction confirmed that all solids precipitated within the SDF groups were crystalline and that there was a positive correlation between the increased percentage of crystal size and the concentration of SDF. The powder X-ray diffraction patterns indicated that fluorohydroxyapatite and silver chloride were formed in all the SDF groups. Compared with calcium phosphate control, a contraction of the unit cell in the a-direction but not the c-direction in SDF groups was revealed, which suggested that small localized fluoride anions substituted the hydroxyl anions in hydroxyapatite crystals. This was further evidenced by the Raman spectra, which displayed up-field shift of the phosphate band in all the SDF groups and confirmed that the chemical environment of the phosphate functionalities indeed changed. The results suggested that SDF reacted with calcium and phosphate ions and produced fluorohydroxyapatite. This preferential precipitation of fluorohydroxyapatite with reduced solubility could be one of the main factors for arrest of caries lesions treated with SDF. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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