A quantitative method for determining a representative detection limit of the forensic luminol test for latent bloodstains.
The luminol test has been used for over 60 years by forensic investigators for presumptive identification of blood and visualization of blood splatter patterns. Multiple studies have estimated the limit of detection (LD) for bloodstains when luminol is employed, with results ranging from 100× to 5,0...
| Publicado en: | Forensic Science International Vol. 278; pp. 396 - 404 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Elsevier B.V.
Sep2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=124934358&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 124934358 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03790738 3L0 jtl: Forensic Science International issn: 03790738 maglogo: N pubinfo: dt: Sep2017 vid: 278 pid: 82545 pub: Elsevier B.V. place: Philadelphia, Pennsylvania artinfo: ui: 124934358 124934358 NLM28837893 10.1016/j.forsciint.2017.06.031 NLM28837893 124934358 ppf: 396 ppct: 8 formats: tig: atl: A quantitative method for determining a representative detection limit of the forensic luminol test for latent bloodstains. aug: au: Cassidy, Brianna M. Lu, Zhenyu Martin, Jennifer P. Tazik, Shawna K. Kellogg, Katie W. DeJong, Stephanie A. Belliveau, Elle O. Kilgore, Katherine E. Ervin, Samantha M. Meece-Rayle, Mackenzie Abraham, Alyssa M. Myrick, Michael L. Morgan, Stephen L. affil: Department of Chemistry and Biochemistry, University of South Carolina, 631 Sumter Street, Columbia, SC 29208, USA sug: subj: Luminescent Measurements Heterocyclic Compounds Indicators and Reagents Videorecording Software Sensitivity and Specificity Forensic Medicine Methods ab: The luminol test has been used for over 60 years by forensic investigators for presumptive identification of blood and visualization of blood splatter patterns. Multiple studies have estimated the limit of detection (LD) for bloodstains when luminol is employed, with results ranging from 100× to 5,000,000× dilute. However, these studies typically have not identified and controlled important experimental variables which may affect the luminol LD for bloodstains. Without control of experimental parameters in the laboratory, variables which affect the potential of presumptive bloodstain test methods remain largely unknown, and comparisons required to establish new, more powerful detection methods are simply impossible. We have developed a quantitative method to determine the relationship between the amount of blood present and its reaction with luminol by measuring, under controlled conditions, the resulting chemiluminescent intensity with a video camera, combined with processing of the digital intensity data. The method resulted in an estimated LD for bloodstains on cotton fabric at ∼200,000× diluted blood with a specific luminol formulation. Although luminol is the focus of this study, the experimental protocol used could be modified to study effects of variables using other blood detection reagents. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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