Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes.
Background/purpose: Real-time quantitative polymerase chain reaction (RQ-PCR) for fusion transcripts and flow cytometry for leukemia-specific markers are widely used for minimal residual disease (MRD) detection in acute lymphoblastic leukemia, but the relation between the results of either method is...
| Publicado en: | Journal of the Formosan Medical Association Vol. 116; no. 10; pp. 774 - 782 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Elsevier B.V.
Oct2017
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125175919&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125175919 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09296646 3O8P jtl: Journal of the Formosan Medical Association issn: 09296646 maglogo: N pubinfo: dt: Oct2017 vid: 116 iid: 10 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 125175919 125175919 NLM28063722 10.1016/j.jfma.2016.12.002 NLM28063722 125175919 ppf: 774 ppct: 8 formats: tig: atl: Concordance of two approaches in monitoring of minimal residual disease in B-precursor acute lymphoblastic leukemia: Fusion transcripts and leukemia-associated immunophenotypes. aug: au: Huang, Ying-Jung Coustan-Smith, Elaine Kao, Hsiao-Wen Liu, Hsi-Che Chen, Shih-Hsiang Hsiao, Chih-Cheng Yang, Chao-Ping Jaing, Tang-Her Yeh, Ting-Chi Kuo, Ming-Chung Lai, Chang-Liang Chang, Chia-Hui Campana, Dario Liang, Der-Cherng Shih, Lee-Yung affil: Division of Hematology-Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan, Taiwan sug: subj: Neoplastic Processes Pathology Leukemia, Lymphocytic, Acute Diagnosis Proteins Leukemia, Lymphocytic, Acute Regression Immunophenotyping Acute Disease Bone Marrow Pathology Flow Cytometry Polymerase Chain Reaction Questionnaires ab: Background/purpose: Real-time quantitative polymerase chain reaction (RQ-PCR) for fusion transcripts and flow cytometry for leukemia-specific markers are widely used for minimal residual disease (MRD) detection in acute lymphoblastic leukemia, but the relation between the results of either method is unclear.Methods: Mononucleated cells from 108 bone marrow samples collected from 55 B-precursor acute lymphoblastic leukemia patients (30 with t(12;21)/ETV6-RUNX1, 16 with t(9;22)/BCR-ABL1 and nine with t(1;19)/TCF3-PBX1) were examined in tandem by RQ-PCR and six-color flow cytometry.Results: MRD results were concordant in 91 of the 108 paired samples (84.2%; K=0.690); 49 samples were MRD-negative while 42 were MRD-positive by both methods, with < 1 log difference in positive MRD estimates in 39 samples (92.9%). Of the 17 discordant samples, 16 were MRD-positive by RQ-PCR but MRD-negative by flow cytometry; the opposite was true in one sample. Kappa value/concordance was 0.690/85.0% (n = 60) for ETV6-RUNX1, 0.842/93.3% (n = 15) for TCF3-PBX1, and 0.535/78.8% (n = 33) for BCR-ABL1. Specific immunophenotypic abnormalities were more prevalent in each genetic subgroup, such as CD38 underexpression, CD58 overexpression, and CD34 overexpression in ETV6-RUNX1, TCF3-PBX1, and BCR-ABL1, respectively.Conclusion: In most follow-up samples, MRD estimates by two methods are in agreement, especially in patients with TCF3-PBX1. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|