Cloning of Helicobacter suis cholesterol α-glucosyltransferase and production of an antibody capable of detecting it in formalin-fixed, paraffin-embedded gastric tissue sections.
Helicobacter suis ( H. suis), formerly called Helicobacter heilmannii type 1 ( H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here,...
| Publicado en: | Histochemistry & Cell Biology Vol. 148; no. 4; pp. 463 - 472 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Oct2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125186984&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125186984 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Oct2017 vid: 148 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 125186984 143959014 10.1007/s00418-017-1582-4 125186984 ppf: 463 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Cloning of Helicobacter suis cholesterol α-glucosyltransferase and production of an antibody capable of detecting it in formalin-fixed, paraffin-embedded gastric tissue sections. aug: au: Kawakubo, Masatomo Horiuchi, Kazuki Komura, Hitomi Sato, Yoshiko Kato, Masayoshi Ikeyama, Meguru Fukushima, Mana Yamada, Shigenori Ishizone, Satoshi Matsumoto, Takehisa Ota, Hiroyoshi Sagara, Junji Nakayama, Jun affil: Department of Molecular Pathology , Shinshu University Graduate School of Medicine , Matsumoto 390-8621 Japan sug: ab: Helicobacter suis ( H. suis), formerly called Helicobacter heilmannii type 1 ( H. heilmannii), is a gram-negative bacterium of the Helicobacter species. This pathogen infects the stomach of humans and animals such as dogs, cats, pigs, and rodents, the latter giving rise to zoonotic infection. Here, we generated a H. suis-specific antibody useful for immunohistochemistry with formalin-fixed, paraffin-embedded tissue sections. To do so, we began by cloning the gene encoding H. suis cholesterol α-glucosyltransferase (αCgT). αCgT is the key enzyme responsible for biosynthesis of cholesteryl α- d-glucopyranoside (CGL), a major cell wall component of Helicobacter species including H. suis. The deduced amino acid sequence of H. suis αCgT had 56% identity with the corresponding Helicobacter pylori ( H. pylori). We then developed a polyclonal antibody (anti-Hh-I205R) by immunizing rabbits with a 205 amino acid H. suis αCgT fragment. Immunohistochemistry with the anti-Hh-I205R antibody could differentiate H. suis from H. pylori in gastric mucosa sections derived from mice infected with either pathogen. We then probed formalin-fixed, paraffin-embedded sections of human gastric mucosa positive for H. suis infection with the anti-Hh-I205R antibody and detected positive staining. These results indicate that anti-Hh-I205R antibody is specific for H. suis αCgT and useful to detect H. suis in gastric specimens routinely analyzed in pathological examinations. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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