A flexible cell concentrator using inertial focusing.
Cell concentration adjustment is intensively implemented routinely both in research and clinical laboratories. Centrifuge is the most prevalent technique for tuning biosample concentration. But it suffers from a number of drawbacks, such as requirement of experienced operator, high cost, low resolut...
| Publicado en: | Biomedical Microdevices Vol. 19; no. 4; pp. 1 - 13 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Dec2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125294505&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125294505 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Dec2017 vid: 19 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 125294505 10.1007/s10544-017-0223-y 125294505 ppf: 1 ppct: 12 formats: fmt: @attributes: type: P tig: atl: A flexible cell concentrator using inertial focusing. aug: au: Tu, Chunglong Zhou, Jian Liang, Yitao Huang, Bobo Fang, Yifeng Liang, Xiao Ye, Xuesong affil: Department of General Surgery, Sir Run Run Shaw Hospital, College of Medicine , Zhejiang University , Hangzhou 310016 China sug: ab: Cell concentration adjustment is intensively implemented routinely both in research and clinical laboratories. Centrifuge is the most prevalent technique for tuning biosample concentration. But it suffers from a number of drawbacks, such as requirement of experienced operator, high cost, low resolution, variable reproducibility and induced damage to sample. Herein we report on a cost-efficient alternative using inertial microfluidics. While the majority of existing literatures concentrate on inertial focusing itself, we identify the substantial role of the outlet system played in the device performance that has long been underestimated. The resistances of the outlets virtually involve in defining the cutoff size of a given inertial filtration channel. Following the comprehensive exploration of the influence of outlet system, we designed an inertial device with selectable outlets. Using both commercial microparticles and cultured Hep G2 cells, we have successfully demonstrated the automated concentration modification and observed several key advantages of our device as compared with conventional centrifuge, such as significantly reduced cell loss (only 4.2% vs. ~40% of centrifuge), better preservation of cell viability and less processing time as well as the increased reproducibility due to absence of manual operation. Furthermore, our device shows high effectiveness for concentrated sample (e.g., 1.8 × 10 cells/ml) as well. We envision its promising applications in the circumstance where repetitive sample preparation is intensely employed. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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