Vitamin D-Loaded Nanostructured Lipid Carrier (NLC): A New Strategy for Enhancing Efficacy of Doxorubicin in Breast Cancer Treatment.
The dangerous dose-dependent side effects of anticancer agents triggered the finding of new approaches for elevated chemotherapy efficacy. This study investigated the potential application of nanostructured lipid careers (NLCs) in increasing vitamin D3 (VitD) effectiveness in breast cancer cell (MCF...
| Publicado en: | Nutrition & Cancer Vol. 69; no. 6; pp. 840 - 849 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Aug/Sep2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125544289&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125544289 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Aug/Sep2017 vid: 69 iid: 6 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 125544289 125544289 125544289 10.1080/01635581.2017.1339820 125544289 ppf: 840 ppct: 9 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Vitamin D-Loaded Nanostructured Lipid Carrier (NLC): A New Strategy for Enhancing Efficacy of Doxorubicin in Breast Cancer Treatment. aug: au: Sabzichi, Mehdi Mohammadian, Jamal Mohammadi, Maryam Jahanfar, Farhad Movassagh Pour, Ali Akbar Hamishehkar, Hamed Ostad-Rahimi, Alireza affil: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran sug: subj: Breast Neoplasms Prevention and Control Doxorubicin Pharmacodynamics Cholecalciferol Pharmacodynamics Nanostructures Utilization Human In Vitro Studies Models, Biological Drug Combinations Pharmacodynamics Spectrum Analysis Microscopy Biological Assay Staining and Labeling Flow Cytometry Polymerase Chain Reaction Descriptive Statistics Dose-Response Relationship, Drug Cell Proliferation Drug Effects P-Value Apoptosis Drug Effects Gene Expression Evaluation Statistical Significance Drug Synergism ab: The dangerous dose-dependent side effects of anticancer agents triggered the finding of new approaches for elevated chemotherapy efficacy. This study investigated the potential application of nanostructured lipid careers (NLCs) in increasing vitamin D3 (VitD) effectiveness in breast cancer cell (MCF-7) in concurrent administration with doxorubicin (Dox). VitD-loaded NLCs were characterized by particle size, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscope. Cytotoxicity and molecular effects of formulation were evaluated by MTT, DAPI staining, flow cytometry, and real-time quantitative PCR assays. The formulation revealed mean particle size of 87±5 nm with a polydispersity index of 0.24 confirmed by SEM images. The IC50 values for VitD and Dox were 1.3 ± 0.04 and 0.65 ± 0.05 µM, respectively. VitD-loaded NLCs decreased the percentage of cell proliferation from 49 ± 7.2% to 37 ± 5.1% (P < 0.05). Cotreatment of the cells with VitD-loaded NLCs and Dox caused over a twofold increase in the percentage of apoptosis (P < 0.05). Gene expression profile demonstrated a significant decrease in antiapoptotic factor survivin along with increase in proapoptotic factor Bax mRNA levels. Overall, our results introduced the NLC technology as a novel strategy to elevate the efficacy of chemotherapeutics in breast cancer. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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