Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains.
Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). T...
| Publicado en: | BioMed Research International Vol. 2017; pp. 1 - 8 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
10/11/2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125597008&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125597008 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 10/11/2017 vid: 2017 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 125597008 125597008 125597008 10.1155/2017/1762162 125597008 ppf: 1 ppct: 7 formats: fmt: @attributes: type: P tig: atl: Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains. aug: au: Sellyei, Boglárka Bányai, Krisztián Bartha, Dániel Hajtós, István Fodor, László Makrai, László affil: CAR, HAS, Institute for Veterinary Medical Research, P.O. Box 18, Budapest 1581, Hungary sug: subj: Corynebacterium Infections Familial and Genetic Sequence Analysis Human Hungary Genetic Techniques Polymerase Chain Reaction ab: Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). The in silico analysis of the 16 frequently studied housekeeping genes showed that C. pseudotuberculosis strains could be readily distinguished from C. diphtheriae and C. ulcerans strains; however, sequences of the same genes in the two biotypes of the C. pseudotuberculosis were highly similar; the heterogeneity values were low. Genes dnaK, infB, groEL1, and leuA showed marked genetic variation within C. pseudotuberculosis, and strains of the two biotypes of C. pseudotuberculosis could be differentiated. Analysis of the individual genes showed a fairly conservative nature of C. pseudotuberculosis biotype ovis strains. The greatest genetic differentiation was seen in the dnaK and infB genes and concatenations of these two genes were very useful in the genetic separation of the studied strains. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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