کلونسازی و بررسی بیان ژن ureG به عنوان کاندیدای واکسن ژنی علیه هلیکوباکترپیلوری
Introduction: Helicobacter pylori is a gram-negative bacterium that has infected many human societies worldwide. the urease of H. pylori is essential for its survival in the human stomach and the ureG gene is one of the most important virulence factors. Objective: The aim of this study is the clonin...
| Publicado en: | Journal of Guilan University of Medical Sciences Vol. 26; no. 102; pp. 20 - 30 |
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| Autores principales: | , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Journal of Guilan University of Medical Sciences
2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125731938&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125731938 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20084048 EVAI jtl: Journal of Guilan University of Medical Sciences issn: 20084048 maglogo: N pubinfo: dt: 2017 vid: 26 iid: 102 pid: 76615 pub: Journal of Guilan University of Medical Sciences artinfo: ui: 125731938 125731938 125731938 125731938 ppf: 20 ppct: 10 formats: tig: atl: کلونسازی و بررسی بیان ژن ureG به عنوان کاندیدای واکسن ژنی علیه هلیکوباکترپیلوری aug: au: محمودی واشیان, زهرا دوستی, عباس affil: مركز تحقيقات بيوتكنولوژي، واحد شهركرد، دانشگاه آزاد اسلامي، شهركرد، ايران sug: subj: Genetic Techniques DNA Gene Expression Helicobacter Pylori Immunology Vaccines Urease Polymerase Chain Reaction Gene Amplification Experimental Studies ab: Introduction: Helicobacter pylori is a gram-negative bacterium that has infected many human societies worldwide. the urease of H. pylori is essential for its survival in the human stomach and the ureG gene is one of the most important virulence factors. Objective: The aim of this study is the cloning of the ureG gene in order to generate a gene vaccine against H. pylori. Materials and Methods: In this experimental study, the DNA was extracted from helicobacter pylori. amplification of ureG gene was performed using specific primers and the PCR products were cloned into pTZ vector using T/A cloning technique. the ureG gene was then cut from the pTZ vector using the XbaI and SalI enzymes and the gene was subcloned in the pCI-neo expression vector. the pCI-neo-ureG recombinant vector was transformed into CHO cells by electroporation, and ureG gene expression was detected on a SDS-PAGE gel. Results: The H. pylori ureG gene was amplified and isolated by PCR successfully. the results showed that the ureG gene was cloned into pTZ and pCI-neo vectors, correctly and the pCI-neo-ureG final construct was generated. the results of insertion of final construct into CHO cells showed the 23 KDa product on SDS-PAGE gel. Conclusion: The ureG gene cloned into pCI-neo expression vector has the potency of expression and production of the specific product of this gene in CHO animal cell. therefore, this gene construct can be used as a suitable candidate for the further experiments of recombinant vaccines against H. pylori in animal models. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: Persian refInfo: holdings: @attributes: islocal: N |
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