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Bacterial Biofilm Assay for the Clinical Laboratory.

Biofilms are microbial communities attached to a surface, resistant to antimicrobic agents, and causative of medical device-associated and chronic infections. To date, no clinical laboratory test has been identified to assist in diagnosis of biofilm-associated infection. For that reason, this study...

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Published in:Clinical Laboratory Science Vol. 30; no. 3; pp. 155 - 161
Main Authors: SYSKIN, EKATERINA, TRAN, LAURA, DUESSEL, SHARON, HEUERTZ, RITA M.
Format: research tables/charts Journal Article
Published: American Society for Clinical Laboratory Science Summer2017
Online Access:View this record in EBSCOhost
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      dt: Summer2017
      vid: 30
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      pub: American Society for Clinical Laboratory Science
      place: Dundee, Michigan
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        atl: Bacterial Biofilm Assay for the Clinical Laboratory.
      aug:
        au:
          SYSKIN, EKATERINA
          TRAN, LAURA
          DUESSEL, SHARON
          HEUERTZ, RITA M.
        affil: Department of Biomedical Laboratory Science, Doisy College of Health Sciences, Saint Louis University, St. Louis, MO
      sug:
        subj:
          Biofilms
          Laboratory Developed Tests
          Gram-Negative Bacterial Infections Diagnosis
          Gram-Positive Bacterial Infections Diagnosis
          Gram-Positive Bacterial Infections Etiology
          Gram-Negative Bacterial Infections Etiology
          Pseudomonas
          Staphylococcus Aureus
          Methicillin-Resistant Staphylococcus Aureus
          In Vitro Studies
          Dyes
          Human
          Funding Source
      ab: Biofilms are microbial communities attached to a surface, resistant to antimicrobic agents, and causative of medical device-associated and chronic infections. To date, no clinical laboratory test has been identified to assist in diagnosis of biofilm-associated infection. For that reason, this study focused on assay development. Two model systems of bacteria, Staphylococcus aureus and Pseudomonas aeruginosa (Gram positive and Gram negative, respectively) were used for assay development since these organisms frequently are able to produce biofilms and cause antimicrobic-resistant infections. Test format was growth of bacteria in a 96-well microplate using reagents and equipment readily available in clinical laboratories. Procedural steps included inoculation of isolated bacteria into broth and standardization of bacterial number using spectrophotometer or McFarland density standard comparison. Bacteria were dispensed into microplate wells, incubated to allow for biofilm formation; biofilm was then stained, solubilized and quantitated by spectrophotometry. Results indicated that bacterial biofilm is quantifiable using the assay herein defined. In addition, S. aureus and P. aeruginosa are able to produce large amounts of biofilm; and MRSA produces more biofilm than MSSA.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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