Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines.
Parasite infections cause dramatic anatomical and ultrastructural changes in host plants. Cyst nematodes are parasites that invade host roots and induce a specific feeding structure called a syncytium. A syncytium is a large multinucleate cell formed by cell wall dissolution-mediated cell fusion. Th...
| Publicado en: | Protoplasma Vol. 254; no. 6; pp. 2107 - 2116 |
|---|---|
| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Nov2017
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=125839855&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 125839855 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Nov2017 vid: 254 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 125839855 144108054 10.1007/s00709-017-1105-0 125839855 ppf: 2107 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Spatiotemporal deep imaging of syncytium induced by the soybean cyst nematode Heterodera glycines. aug: au: Ohtsu, Mina Sato, Yoshikatsu Kurihara, Daisuke Suzaki, Takuya Kawaguchi, Masayoshi Maruyama, Daisuke Higashiyama, Tetsuya affil: Graduate School of Science , Nagoya University , Furo-cho, Chikusa-ku Nagoya 464-8602 Japan sug: ab: Parasite infections cause dramatic anatomical and ultrastructural changes in host plants. Cyst nematodes are parasites that invade host roots and induce a specific feeding structure called a syncytium. A syncytium is a large multinucleate cell formed by cell wall dissolution-mediated cell fusion. The soybean cyst nematode (SCN), Heterodera glycines, is a major soybean pathogen. To investigate SCN infection and the syncytium structure, we established an in planta deep imaging system using a clearing solution ClearSee and two-photon excitation microscopy (2PEM). Using this system, we found that several cells were incorporated into the syncytium; the nuclei increased in size and the cell wall openings began to be visible at 2 days after inoculation (DAI). Moreover, at 14 DAI, in the syncytium developed in the cortex, there were thickened concave cell wall pillars that resembled 'Parthenon pillars.' In contrast, there were many thick board-like cell walls and rarely Parthenon pillars in the syncytium developed in the stele. We revealed that the syncytia were classified into two types based on the pattern of the cell wall structures, which appeared to be determined by the position of the syncytium inside roots. Our results provide new insights into the developmental process of syncytium induced by cyst nematode and a better understanding of the three-dimensional structure of the syncytium in host roots. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|