MiR-375 and YAP1 expression profiling in medullary thyroid carcinoma and their correlation with clinical-pathological features and outcome.

Medullary thyroid cancer (MTC) is a tumor marked by an indolent growth for which few prognostic factors and therapeutic strategies are actually available. Different studies have recently appraised well-differentiated thyroid cancers are characterized by a dysregulation in different microRNA sets; ho...

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Publicado en:Virchows Archiv: European Journal of Pathology Vol. 471; no. 5; pp. 651 - 659
Autores principales: Galuppini, Francesca, Bertazza, Loris, Barollo, Susi, Cavedon, Elisabetta, Rugge, Massimo, Guzzardo, Vincenza, Sacchi, Diana, Watutantrige-Fernando, Sara, Vianello, Federica, Mian, Caterina, Pennelli, Gianmaria
Formato: pictorial research tables/charts Journal Article
Publicado: Springer Nature Nov2017
Acceso en línea:Ver este registro en EBSCOhost
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        atl: MiR-375 and YAP1 expression profiling in medullary thyroid carcinoma and their correlation with clinical-pathological features and outcome.
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          Galuppini, Francesca
          Bertazza, Loris
          Barollo, Susi
          Cavedon, Elisabetta
          Rugge, Massimo
          Guzzardo, Vincenza
          Sacchi, Diana
          Watutantrige-Fernando, Sara
          Vianello, Federica
          Mian, Caterina
          Pennelli, Gianmaria
        affil: Department of Medicine (DIMED), Surgical Pathology Unit , University of Padova , Via Gabelli 61 35121 Padova Italy
      sug:
        subj:
          Neuroendocrine Tumors Pathology
          Carrier Proteins
          RNA
          Phosphoproteins
          Thyroid Neoplasms Pathology
          Child
          Young Adult
          Carrier Proteins Analysis
          Adolescence
          RNA Analysis
          Gene Expression Profiling
          Aged
          Male
          Adult
          Female
          Child, Preschool
          Aged, 80 and Over
          Phosphoproteins Analysis
          Middle Age
          Human
          Child: 6-12 years
          Adolescent: 13-18 years
          Aged: 65+ years
          Adult: 19-44 years
          Child, Preschool: 2-5 years
          Aged, 80 & over
          Middle Aged: 45-64 years
          Male
          Female
      ab: Medullary thyroid cancer (MTC) is a tumor marked by an indolent growth for which few prognostic factors and therapeutic strategies are actually available. Different studies have recently appraised well-differentiated thyroid cancers are characterized by a dysregulation in different microRNA sets; however, only few of them investigated the role of miRNA expression in MTCs. In this study, we have assessed the miR-375 expression in a series of 130 MTCs (104 are sporadic and 26 familial) with a median follow-up of 39 months (range 1-138) and then we have correlated our results with the clinical-pathological features and the patients' outcome.Moreover, we have appraised YAP1 (Yes-associated protein 1) immunohistochemical expression in the same MTC series and in 5 C-cells hyperplasia (CCH) samples as well. We observed a significant upregulation of miR-375 in all MTCs, when compared to the normal thyroid tissues. Besides, miR-375 expression was found to be closely linked to neoplastic size, a chance of thyroid capsule infiltration, the risk of lymph node metastasis, and the staging of the tumor. At the end of follow-up, only 10% (13/130) showed a tumor progression and a higher miR-375 expression was found to be closely linked to a worst patient' outcome. On the contrary, YAP1 immunohistochemical expression was sharply downregulated in tumors, whereas it was weakly expressed in CCHs. Our results suggest miR-375 plays a central role in MTC progression and, therefore, we seek following the idea that miR-375 pathway may be an effective target in novel MTC therapeutic strategies.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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