بررسی اثر مدت زمان انکوباسیون و تیمار ویتامین E بر تحرک ، حیات و فراگمنتاسیون DNA اسپرم نمونه های آستنوتراتوزواسپرمی
Background: In Asthenoteratozoospermic men, low motility, defected DNA and highly oxidative stress in sperm cause poor assisted reproductive techniques (ART) outcomes. The aim of this study was to determine the effect of Vitamin E (Vit E), as a potent antioxidant, on sperm motility, viability and DN...
| Publicado en: | Arak Medical University Journal Vol. 20; no. 6; pp. 62 - 73 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Arak University of Medical Sciences
2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=126068019&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 126068019 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17355338 B2BM jtl: Arak Medical University Journal issn: 17355338 maglogo: N pubinfo: dt: 2017 vid: 20 iid: 6 pid: 59841 pub: Arak University of Medical Sciences artinfo: ui: 126068019 126068019 126068019 126068019 ppf: 62 ppct: 11 formats: tig: atl: بررسی اثر مدت زمان انکوباسیون و تیمار ویتامین E بر تحرک ، حیات و فراگمنتاسیون DNA اسپرم نمونه های آستنوتراتوزواسپرمی aug: au: غفاری زاده, علی اصغر واعظی, غلامحسن شریعت زاده, محمد علی ملکی راد, علی اکبر affil: دانشجوي دکتري تخصصی فیزیولوژي، گروه زیست شناسی، دانشکده علوم پایه، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران sug: subj: Vitamin E Administration and Dosage Sperm Motility Evaluation Male Human Semen Analysis In Vitro Studies Oxidative Stress Male ab: Background: In Asthenoteratozoospermic men, low motility, defected DNA and highly oxidative stress in sperm cause poor assisted reproductive techniques (ART) outcomes. The aim of this study was to determine the effect of Vitamin E (Vit E), as a potent antioxidant, on sperm motility, viability and DNA integrity at different times of in vitro incubation (after 2, 4 and 6-h) to improve asthenoteratozoospermic semen samples for ART. Materials and Methods: Asthenoteratozoospermic semen samples of 50 volunteers were collected and examined. Each sample was divided into two groups of control and vitamin E (2mM) and kept in the 37 °C and 6 % CO2 for 2, 4 and 6 hours. After this incubation, sperm motility, viability and sperm DNA fragmentation (SCD) were evaluated in each group. Data were analyzed using repeated measurement of ANOVA and T-test. The means were considered significantly different at p<0.05. Results:Significant decrease in total and progressive motility and viability as well as significant increase in sperm DNA damage (after 6h of incubation) were found in control group vs. the control group before incubation (p<0.05). The sperm motility and viability was significantly higher in vitamin E group compared to untreated control group (p<0.05). Our results also showed that DNA fragmentation significantly was lower after 6h of vitamin E treatment (p<0.05). Conclusion: In vitro supplementation of vitamin E in asthenoteratozoospermia semen samples may protect spermatozoa from maltreatment effect of ROS during sperm sampling via keeping enzymatic and antioxidant process in optimum condition. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: Persian refInfo: holdings: @attributes: islocal: N |
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