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Connexin43 and AMPK Have Essential Role in Resistance to Oxidative Stress Induced Necrosis.

Reactive oxygen species (ROS) induced oxidative stress leads to cell damage and neurological disorders in astrocytes. The gap junction protein connexin43 (Cx43) could form intercellular channels in astrocytes and the expression of Cx43 plays an important role in protecting the cells from damage. In...

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Published in:BioMed Research International Vol. 2017; pp. 1 - 8
Main Authors: Zhao, Chunshan, Fang, Jinnv, Li, Chunguo, Zhang, Min
Format: pictorial research tables/charts Journal Article
Published: Wiley-Blackwell 11/27/2017
Online Access:View this record in EBSCOhost
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        23146133
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      jtl: BioMed Research International
      issn: 23146133
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      dt: 11/27/2017
      vid: 2017
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        126426182
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        10.1155/2017/3962173
        126426182
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      tig:
        atl: Connexin43 and AMPK Have Essential Role in Resistance to Oxidative Stress Induced Necrosis.
      aug:
        au:
          Zhao, Chunshan
          Fang, Jinnv
          Li, Chunguo
          Zhang, Min
        affil: Beihua University, Jilin, Jilin, China
      sug:
        subj:
          Connexins
          Central Nervous System Pathology
          Cells Pathology
          Necrosis
          Hydrogen Peroxide Pharmacodynamics
          Protein Kinases Metabolism
          Animal Studies
          In Vitro Studies
          Models, Biological
          Microscopy Methods
          Flow Cytometry
          Blotting, Western
          Apoptosis Evaluation
          Proteins Metabolism
          Reactive Oxygen Species Metabolism
          Signal Transduction
      ab: Reactive oxygen species (ROS) induced oxidative stress leads to cell damage and neurological disorders in astrocytes. The gap junction protein connexin43 (Cx43) could form intercellular channels in astrocytes and the expression of Cx43 plays an important role in protecting the cells from damage. In the present study, we investigated the contribution of Cx43 to astrocytic necrosis induced by the ROS hydrogen peroxide (H2O2) and the mechanism by which AMPK was involved in this process. Fluorescence microscopy, flow cytometry, and western blot were used quantitatively and qualitatively to determine the cell apoptosis, necrosis, and protein expression. Lack of Cx43 expression or blockage of Cx43 channels resulted in increased H2O2-induced astrocytic necrosis, supporting a cell protective effect of functional Cx43 channels. Our data suggest that AMPK is important for Cx43-mediated ROS resistance. Inhibition of AMPK activation results in reduction of necrosis and ROS production. Taken together, our findings suggest that the role of Cx43 in response to H2O2 stress is dependent on the activation of AMPK signaling pathways and regulates ROS production and cell necrosis.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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