Cocaine- and amphetamine-regulated transcript (CART) peptide in the nerve fibres of the porcine gallbladder wall under physiological conditions and after Salmonella Enteritidis lipopolysaccharides administration.

Background: Cocaine- and amphetamine-regulated transcript (CART) peptide is the substance distributed in various parts of the nervous system. The majority of previous studies described this substance in the brain, where it takes part in regulatory processes connected with the food intake. CART is al...

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Publicado en:Folia Morphologica Vol. 76; no. 4; pp. 596 - 603
Autores principales: Mikolajczyk, A., Makowska, K., Mikołajczyk, Anita, Makowska, Krystyna, Mikołajczyk, A
Formato: Journal Article
Publicado: VM Medica-VM Group (Via Medica) 2017
Acceso en línea:Ver este registro en EBSCOhost
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      pub: VM Medica-VM Group (Via Medica)
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        atl: Cocaine- and amphetamine-regulated transcript (CART) peptide in the nerve fibres of the porcine gallbladder wall under physiological conditions and after Salmonella Enteritidis lipopolysaccharides administration.
      aug:
        au:
          Mikolajczyk, A.
          Makowska, K.
          Mikołajczyk, Anita
          Makowska, Krystyna
          Mikołajczyk, A
        affil: Department of Public Health, Epidemiology and Microbiology, Faculty of Medical Sciences, University of Warmia and Mazury, Olsztyn, Poland
      sug:
      ab: Background: Cocaine- and amphetamine-regulated transcript (CART) peptide is the substance distributed in various parts of the nervous system. The majority of previous studies described this substance in the brain, where it takes part in regulatory processes connected with the food intake. CART is also present in the peripheral nervous system, especially in the enteric neurons and nerves located in the wall of the stomach and intestine, but many aspects of distribution and functions of this peptide in the digestive organs remain unknown. The aim of the present study was to investigate the changes of CART-positive nerves in the porcine gallbladder after administration of low-dose Salmonella Enteritidis lipopolysaccharide (LPS) using the single immunofluorescence technique.Materials and Methods: Seven days after the injection of 5 μg/kg b.w. LPS S. Enteritidis the gallbladders were collected. CART-positive nerves were studied with standard single immunofluorescence method and counted per observation field (0.1 m2).Results: In control animals the average number of CART-positive nerves per observation field (0.1 mm2) amounted to 5.38 ± 0.32, 11.11 ± 1.56 and 2.97 ± ± 0.24 in gallbladder neck, body and fundus, respectively. LPS administration caused the increase in the number of CART-positive fibres in all parts of gallbladder, and these values amounted to 12.74 ± 0.51, 19.75 ± 0.19 and 5.1 ± 0.05 in the gallbladder neck, body and fundus, respectively.Conclusions: The obtained results suggest that CART is involved in the neuronal regulatory processes in the porcine gallbladder under physiological conditions, but also during pathological processes, but exact functions of this peptide in this organ remain unexplained and require the further investigation.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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