Mitochondrial Imaging with Combined Fluorescence and Stimulated Raman Scattering Microscopy Using a Probe of the Aggregation-Induced Emission Characteristic.
In vivo quantitative measurement of biodistri-bution plays a critical role in the drug/probe development and diagnosis/treatment process monitoring. In this work, we report a probe, named AIE-SRS-Mito, for imaging mitochondria in live cells via fluorescence (FL) and stimulated Raman scattering (SRS)...
| Published in: | Journal of the American Chemical Society Vol. 139; no. 47; pp. 17022 - 17031 |
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| Main Authors: | , , , , |
| Format: | Article |
| Published: |
American Chemical Society
11/29/2017
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| Subjects: | |
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=126609373&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 126609373 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 11/29/2017 vid: 139 iid: 47 pid: 997 pub: American Chemical Society artinfo: ui: 126609373 10.1021/jacs.7b06273 ppf: 17022 ppct: 9 formats: tig: atl: Mitochondrial Imaging with Combined Fluorescence and Stimulated Raman Scattering Microscopy Using a Probe of the Aggregation-Induced Emission Characteristic. aug: au: Xuesong Li Meijuan Jiang Lam, Jacky W. Y. Ben Zhong Tang Qu, Jianan Y. affil: Department of Electronic and Computer Engineering, School of Science, and Institute for Advanced Study Center of Systems Biology and Human Health, School of Science, and Institute for Advanced Study Department of Chemistry, Hong Kong Branch of the Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, and Division of Biomedical Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong China su: Mitochondrial pathology Imaging systems Photoluminescence Raman scattering Nanostructured materials sug: subj: Mitochondrial pathology Imaging systems Photoluminescence Raman scattering Nanostructured materials ab: In vivo quantitative measurement of biodistri-bution plays a critical role in the drug/probe development and diagnosis/treatment process monitoring. In this work, we report a probe, named AIE-SRS-Mito, for imaging mitochondria in live cells via fluorescence (FL) and stimulated Raman scattering (SRS) imaging. The probe features an aggregation-induced emission (AIE) characteristic and possesses an enhanced alkyne Raman peak at 2223 cm. The dual-mode imaging of AIE-SRS-Mito for selective mitochondrion-targeting was examined on a homemade FL-SRS microscope system. The detection limit of the probe in the SRS imaging was estimated to be 8.5 μM. Due to the linear concentration dependence of SRS and inertness of the alkyne Raman signal to environmental changes, the intracellular distribution of the probe was studied, showing a local concentration of >2.0 mM in the mitochondria matrix, which was >100-fold higher than the incubation concentration. To the best of our knowledge, this is the first time that the local concentration of AIE molecules inside cells has been measured noninvasively and directly. Also, the nonquenching effect of such AIE molecules in cell imaging has been verified by the positive correlation of FL and SRS signals. Our work will encourage the utilization of SRS microscopy for quantitative characterization of FL probes or other nonfluorescent compounds in living biological systems and the development of FL-SRS dual-mode probes for specific biotargets. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2017 holdings: @attributes: islocal: N |
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