Evaluation of the Flagging Performance of the Hematology Analyzer Sysmex XN Series on the Basis of ''Q Values''.

Context.--In the XN series of hematology analyzers (Sysmex, Kobe, Japan), the probability of the presence of abnormal cells is indicated by flags based on Q values. Objective.--To evaluate the Q value performance of the Sysmex XN-20 modular analyzer. Design.--The interinstrumental concordance, intra...

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Publicado en:Archives of Pathology & Laboratory Medicine Vol. 142; no. 1; pp. 83 - 89
Autores principales: Oh Joo Kweon, Mi-Kyung Lee, Hye Ryoun Kim
Formato: research tables/charts Journal Article
Publicado: College of American Pathologists Jan2018
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jan2018
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      pub: College of American Pathologists
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        atl: Evaluation of the Flagging Performance of the Hematology Analyzer Sysmex XN Series on the Basis of ''Q Values''.
      aug:
        au:
          Oh Joo Kweon
          Mi-Kyung Lee
          Hye Ryoun Kim
        affil: Department of Laboratory Medicine, Aerospace Medical Center, Republic of Korea Air Force, Cheongju, Republic of Korea
      sug:
        subj:
          Hematology Equipment and Supplies
          Laboratory Equipment and Supplies
          Autoanalyzers
          Lymphocytes
          Precision
          Descriptive Statistics
          Sensitivity and Specificity
          Cell Count
          Human
      ab: Context.--In the XN series of hematology analyzers (Sysmex, Kobe, Japan), the probability of the presence of abnormal cells is indicated by flags based on Q values. Objective.--To evaluate the Q value performance of the Sysmex XN-20 modular analyzer. Design.--The interinstrumental concordance, intrainstrumental precision, and diagnostic accuracy of Q values, with tested flags of ''blasts/abnormal lymphocytes,'' ''atypical lymphocytes,'' and ''blasts,'' were investigated. Results.--Absolute concordance rates in flagging between 2 analyzers ranged from 69.8% to 80.8%, and j values ranged from 0.43 to 0.61. In samples with absolute related cell counts lower than 100/lL, the values ranged from 0.31 to 0.52. For intrainstrumental precision, standard deviations ranged from 4.8 to 23.9 for the blasts/abnormal lymphocytes, from 18.7 to 59.1 for the blasts, and from 11.0 to 23.0 for the atypical lymphocytes. Using a default Q value cutoff, diagnostic accuracy values based on the area under the curve, sensitivity, and specificity were, respectively, 0.910, 90.9%, and 72.2% for blasts/abnormal lymphocytes; 0.927, 84.9%, and 89.8% for blasts; and 0.865, 74.4%, and 84.9% for atypical lymphocytes. The diagnostic accuracy of Q values was much lower in samples with absolute related cell counts lower than 100/lL than in those 100/lL or higher. Conclusions.--Q values of the Sysmex XN-20 analyzer were found to be imprecise and irreproducible, especially with samples containing a small number of pathologic cells. Adjustments in the Q value threshold may help in the detection of these cells.
      pubtype: Academic Journal
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        research
        tables/charts
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      ougenre: Article
    language: English
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