Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae.

A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram-positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urea...

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Publicado en:Molecular Oral Microbiology Vol. 33; no. 1; pp. 105 - 111
Autores principales: Beall, C. J., Mokrzan, E. M., Griffen, A. L., Leys, E. J.
Formato: Journal Article
Publicado: Wiley-Blackwell Feb2018
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: Molecular Oral Microbiology
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      dt: Feb2018
      vid: 33
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        atl: Cultivation of Peptidiphaga gingivicola from subgingival plaque: The first representative of a novel genus of Actinomycetaceae.
      aug:
        au:
          Beall, C. J.
          Mokrzan, E. M.
          Griffen, A. L.
          Leys, E. J.
        affil: Division of Biosciences, The Ohio State University College of Dentistry, Columbus, OH, USA
      sug:
        subj:
          Gram-Positive Bacteria
          Gram-Positive Bacteria Classification
          Periodontal Diseases Microbiology
          Evolution
          Dental Plaque Microbiology
          DNA
          RNA
          Bacterial Typing Techniques
          Phenotype
          Fatty Acids Analysis
          Sequence Analysis
          Immunity
          Biochemical Phenomena
      ab: A novel bacterium was isolated from the subgingival plaque of a patient with periodontal disease. Bacterial strain BA112T is a facultative Gram-positive coccus. It metabolizes alanine, arginine, glycine, histidine, leucine, proline, serine and tyrosine, but does not appear to use carbohydrates. Urease, esculin, indole, catalase and nitrate reduction tests were all negative. Major cellular fatty acids were C18:0 , C12:0 , C16:0 , C18:1 w9c and C20:0 . The genome was sequenced and is 2.4 Mbp in length and has 64% GC content. Based on phylogenetics of the 16S rRNA sequence and concatenated alignments of 37 conserved proteins, BA112T belongs to the family Actinomycetaceae but is located on a branch of the tree without currently named members. Based on our phenotypic and phylogenetic studies, we propose that BA112T is the first known representative of a new genus, for which the name Peptidiphaga gingivicola gen. nov., sp. nov. is proposed. The type strain is BA112T .
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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