FOXP3 T cells are present in kidney biopsy samples in children with tubulointerstitial nephritis and uveitis syndrome.
Background: Tubulointerstitial nephritis (TIN) is an inflammatory disease of unknown pathogenesis. To evaluate a possible role of regulatory T cells (Tregs) in the pathophysiology of TIN with (TINU) and without uveitis, we investigated the presence and quantity of FOXP3 T regulatory lymphocytes in d...
| Publicado en: | Pediatric Nephrology Vol. 33; no. 2; pp. 287 - 294 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Feb2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=127377602&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 127377602 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0931041X EF1 jtl: Pediatric Nephrology issn: 0931041X maglogo: N pubinfo: dt: Feb2018 vid: 33 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 127377602 127377602 144026970 127377602 10.1007/s00467-017-3796-z 127377602 ppf: 287 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: FOXP3 T cells are present in kidney biopsy samples in children with tubulointerstitial nephritis and uveitis syndrome. aug: au: Rytkönen, Sari H. Kulmala, Petri Autio-Harmainen, Helena Arikoski, Pekka Endén, Kira Kataja, Janne Karttunen, Tuomo Nuutinen, Matti Jahnukainen, Timo affil: PEDEGO Research Unit and Medical Research Center (MRC) Oulu, University of Oulu and Oulu University Hospital, Box 23, 90029 OYS, Oulu, Finland sug: subj: Nephritis, Interstitial Diagnosis Uveitis Biopsy T Lymphocytes Physiology Pediatrics Child Nephritis, Interstitial Physiopathology Human Patient Selection Antibodies Immunohistochemistry Proteins Physiology Immunity Staining and Labeling Disease Duration Data Analysis Software Mann-Whitney U Test Child: 6-12 years ab: Background: Tubulointerstitial nephritis (TIN) is an inflammatory disease of unknown pathogenesis. To evaluate a possible role of regulatory T cells (Tregs) in the pathophysiology of TIN with (TINU) and without uveitis, we investigated the presence and quantity of FOXP3 T regulatory lymphocytes in diagnostic kidney biopsies from pediatric patients. Methods: A total of 33 patients (14 TIN and 19 TINU) were enrolled. The quantity of CD4, FOXP3 and double-positive T cells in formalin-fixed kidney biopsies was determined using double label immunohistochemistry with anti-human CD4 and FOXP3 antibodies. Results: FOXP3 staining was successful in all 33 patients. In patients with chronic uveitis, the density of FOXP3 cells was significantly lower ( p = 0.046) than in TIN patients without uveitis or with uveitis lasting <3 months. CD4 staining was successful in 23 patients. The density of all lymphocytes (CD4, CD4FOXP3 and FOXP3 cells) was significantly lower ( p = 0.023) in patients with chronic uveitis than in other patients. Conclusions: FOXP3 T cells are present in kidney biopsy samples from TIN and TINU patients. In patients with chronic uveitis, the density of FOXP3 T cells is significantly lower than in other patients, suggesting a different pathomechanism for these clinical conditions. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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