Metagenomics in pediatrics: using a shotgun approach to diagnose infections.
Purpose Of Review: With the advent of novel massively parallel sequencing technologies and bioinformatic processing capabilities, clinical applications of metagenomic studies are rapidly being integrated into medicine. Through this paper, we hope to introduce this powerful new tool to clinicians car...
| Publicado en: | Current Opinion in Pediatrics Vol. 30; no. 1; pp. 125 - 131 |
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| Autores principales: | , , |
| Formato: | review tables/charts Journal Article |
| Publicado: |
Lippincott Williams & Wilkins
Feb2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=127412173&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 127412173 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10408703 IQY jtl: Current Opinion in Pediatrics issn: 10408703 maglogo: N pubinfo: dt: Feb2018 vid: 30 iid: 1 pid: 433 pub: Lippincott Williams & Wilkins place: Baltimore, Maryland artinfo: ui: 127412173 127412173 NLM29189352 127412173 10.1097/MOP.0000000000000577 NLM29189352 127412173 ppf: 125 ppct: 6 formats: tig: atl: Metagenomics in pediatrics: using a shotgun approach to diagnose infections. aug: au: Bender, Jeffrey M. Bard, Jennifer Dien Dien Bard, Jennifer affil: Division of Infectious Diseases, Department of Pediatrics, Children's Hospital Los Angeles USA sug: subj: Genome Sequence Analysis Infection Diagnosis Infection Microbiology Child Pediatrics Funding Source Child: 6-12 years ab: Purpose Of Review: With the advent of novel massively parallel sequencing technologies and bioinformatic processing capabilities, clinical applications of metagenomic studies are rapidly being integrated into medicine. Through this paper, we hope to introduce this powerful new tool to clinicians caring for children.Recent Findings: Very few studies have looked at metagenomic applications in children. The ability to perform these types of massive sequencing projects was not possible as little as 7 years ago.Summary: Metagenomics is defined as the study of all genetic material within a given sample. Novel sequencing and analysis approaches allow for unbiased assays to identify pathogens missed by targeted sequencing and culture methods. Although not widely available yet, metagenomic studies have been used to diagnose pediatric infections, identify resistance genes in clinical samples, and characterize outbreaks. Although cost and turnaround time have limited its application in clinical laboratories to date, novel platforms and increasing comfort with these techniques continue to push diagnostic metagenomics into clinical pediatric medicine. Much work in this field is yet to be done. That being said, we feel that pediatric clinicians will be using metagenomic techniques in the care of children with increasing frequency in the near future. pubtype: Academic Journal doctype: review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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