Multifractal-based nuclei segmentation in fish images.
The method for nuclei segmentation in fluorescence in-situ hybridization (FISH) images, based on the inverse multifractal analysis (IMFA) is proposed. From the blue channel of the FISH image in RGB format, the matrix of Holder exponents, with one-by-one correspondence with the image pixels, is deter...
| Publicado en: | Biomedical Microdevices Vol. 19; no. 3; pp. 1 - 14 |
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| Autores principales: | , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=127516869&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 127516869 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13872176 ODN jtl: Biomedical Microdevices issn: 13872176 maglogo: N pubinfo: dt: Sep2017 vid: 19 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 127516869 10.1007/s10544-017-0208-x 127516869 ppf: 1 ppct: 13 formats: fmt: @attributes: type: P tig: atl: Multifractal-based nuclei segmentation in fish images. aug: au: Reljin, Nikola Slavkovic-Ilic, Marijeta Tapia, Coya Cihoric, Nikola Stankovic, Srdjan affil: Academic Technology Services, Princeton University, Princeton, NJ, USA. sug: ab: The method for nuclei segmentation in fluorescence in-situ hybridization (FISH) images, based on the inverse multifractal analysis (IMFA) is proposed. From the blue channel of the FISH image in RGB format, the matrix of Holder exponents, with one-by-one correspondence with the image pixels, is determined first. The following semiautomatic procedure is proposed: initial nuclei segmentation is performed automatically from the matrix of Holder exponents by applying predefined hard thresholding; then the user evaluates the result and is able to refine the segmentation by changing the threshold, if necessary. After successful nuclei segmentation, the HER2 (human epidermal growth factor receptor 2) scoring can be determined in usual way: by counting red and green dots within segmented nuclei, and finding their ratio. The IMFA segmentation method is tested over 100 clinical cases, evaluated by skilled pathologist. Testing results show that the new method has advantages compared to already reported methods. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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