Polymethoxylated Flavones from Orange Peels Inhibit Cell Proliferation in a 3D Cell Model of Human Colorectal Cancer.
Polymethoxylated flavones (PMFs) have been recognized to inhibit colorectal cancer proliferation through various mechanisms, however most of these studies have been performed on cells grown as monolayers that present limitations in mimicking the 3D tumor architecture and microenvironment. The main a...
| Publicado en: | Nutrition & Cancer Vol. 70; no. 2; pp. 257 - 267 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Feb/Mar2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=127727753&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 127727753 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01635581 7MS jtl: Nutrition & Cancer issn: 01635581 maglogo: N pubinfo: dt: Feb/Mar2018 vid: 70 iid: 2 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 127727753 127727753 127727753 10.1080/01635581.2018.1412473 127727753 ppf: 257 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Polymethoxylated Flavones from Orange Peels Inhibit Cell Proliferation in a 3D Cell Model of Human Colorectal Cancer. aug: au: Silva, Inês Estrada, Marta F. V. Pereira, Carolina da Silva, Andreia Bento Bronze, Maria R. Alves, Paula M. Duarte, Catarina M.M. Brito, Catarina Serra, Ana Teresa affil: IBET-Instituto de Biologia Experimental e Tecnológica, Oeiras, Portugal sug: subj: Flavones Pharmacodynamics Antineoplastic Agents Pharmacodynamics Colorectal Neoplasms Prevention and Control In Vitro Studies Alternative Therapies Models, Biological Human Cell Proliferation Drug Effects Cell Cycle Drug Effects Apoptosis Drug Effects Descriptive Statistics Dose-Response Relationship, Drug Phenotype Phytochemicals Analysis ab: Polymethoxylated flavones (PMFs) have been recognized to inhibit colorectal cancer proliferation through various mechanisms, however most of these studies have been performed on cells grown as monolayers that present limitations in mimicking the 3D tumor architecture and microenvironment. The main aim of this study was to investigate the anticancer potential of an orange peel extract (OPE) enriched in PMFs in a 3D cell model of colorectal cancer. The OPE was developed by supercritical fluid extraction and the anticancer effect was evaluated in HT29 spheroids cultures in a stirred-tank based system. Results showed that OPE inhibited cell proliferation, induced cell cycle arrest (G2/M phase), promoted apoptosis, and reduced ALDH+population on HT29 spheroids. The antiproliferative activity was significantly lower than that obtained for 2D model (EC50 value of 0.43 ± 0.02 mg/mL) and this effect was dependent on diameter and cell composition/phenotype of spheroids derived from different culture days (day 3 – 0.53 ± 0.05 mg/mL; day 5 – 0.55 ± 0.03 mg/mL; day 7 – 1.24 ± 0.15 mg/mL). HT29 spheroids collected at day 7 presented typical characteristics ofin vivosolid tumors including a necrotic/apoptotic core, hypoxia regions, presence of cancer stem cells, and a less differentiated invasive front. Nobiletin, sinesentin, and tangeretin were identified as the main compounds responsible for the anticancer activity. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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