Isolation and molecular characterization of Cryptococcus species isolated from pigeon nests and Eucalyptus trees.
Background and Purpose: Cryptococcus species are pathogenic and non-pathogenic basidiomycete yeasts that are found widely in the environment. Based on phenotypic methods, this genus has many species; however, its taxonomy is presently being re-evaluated by modern techniques. The Cryptococcus species...
| Publicado en: | Current Medical Mycology Vol. 3; no. 2; pp. 20 - 26 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Mazandaran University of Medical Sciences
Jun2017
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=127809818&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 127809818 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 24233439 JKN0 jtl: Current Medical Mycology issn: 24233439 maglogo: N pubinfo: dt: Jun2017 vid: 3 iid: 2 pid: 45683 pub: Mazandaran University of Medical Sciences artinfo: ui: 127809818 127809818 127809818 10.29252/cmm.3.2.20 127809818 ppf: 20 ppct: 6 formats: tig: atl: Isolation and molecular characterization of Cryptococcus species isolated from pigeon nests and Eucalyptus trees. aug: au: A., Kamari A., Sepahvand R., Mohammadi affil: Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. sug: subj: Cryptococcus Birds Eucalyptus Geographic Factors Genetic Techniques Methods Culture Techniques Methods DNA Culture Media Nucleic Acid Amplification Techniques Methods ab: Background and Purpose: Cryptococcus species are pathogenic and non-pathogenic basidiomycete yeasts that are found widely in the environment. Based on phenotypic methods, this genus has many species; however, its taxonomy is presently being re-evaluated by modern techniques. The Cryptococcus species complex includes two sibling taxa of Cryptococcus neoformans and Cryptococcus gattii. We aimed to investigate the possible distribution of Cryptococcus species in pigeon nests and Eucalyptus trees in Ilam, Iran, using molecular techniques. Materials and Methods: Two hundred and seventy-four specimens were collected from pigeon nests and Eucalyptus trees during 2016-2017. All the specimens were subcultured on Sabouraud Glucose Agar with chloramphenicol and bird seed agar. For molecular identification, the ITS1-5.8S-ITS2 rDNA region was amplified using the first and fourth internal transcribed spacer (ITS1 and ITS4, respectively) primers. The purified products were applied for cycle sequencing reactions in forward direction with ITS1 primer. The obtained results were analyzed with Chromas 2.3. Results: Thirty-three out of 186 cultures (17.7%) and 11 out of 88 cultures (12.5%) were positive among pigeon nest and Eucalyptus tree specimens, respectively. Cryptococcus albidus (17.2%), C. albidus var. kuetzingii (3.4%), C. adeliensis (3.4%), C. uzbekistanensis (3.4%), and C. neoformans var. grubii (3.4%) were isolated from pigeon nests, and Cryptococcus adeliensis (25%) was the only Cryptococcus species isolated from Eucalyptus trees. Conclusion: The presence of pigeons and Eucalyptus trees in the vicinity of some particular places such as rest homes and hospitals should be considered as a risk factor for the immunocompromised population. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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