Absence of DNA double-strand breaks in human peripheral blood mononuclear cells after 3 Tesla magnetic resonance imaging assessed by γH2AX flow cytometry.
Objectives: Magnetic resonance imaging (MRI) is regarded as a non-harming and non-invasive imaging modality with high tissue contrast and almost no side effects. Compared to other cross-sectional imaging modalities, MRI does not use ionising radiation. Recently, however, strong magnetic fields as ap...
| Publicado en: | European Radiology Vol. 28; no. 3; pp. 1149 - 1157 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research tables/charts randomized controlled trial Journal Article |
| Publicado: |
Springer Nature
Mar2018
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=127990355&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 127990355 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09387994 NPH jtl: European Radiology issn: 09387994 maglogo: N pubinfo: dt: Mar2018 vid: 28 iid: 3 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 127990355 127990355 NLM28986695 127990355 10.1007/s00330-017-5056-9 NLM28986695 127990355 ppf: 1149 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Absence of DNA double-strand breaks in human peripheral blood mononuclear cells after 3 Tesla magnetic resonance imaging assessed by γH2AX flow cytometry. aug: au: Fasshauer, Martin Krüwel, Thomas Zapf, Antonia Stahnke, Vera C. Rave-Fränk, Margret Staab, Wieland Sohns, Jan M. Steinmetz, Michael Unterberg-Buchwald, Christina Schuster, Andreas Ritter, Christian Lotz, Joachim affil: Department of Diagnostic and Interventional Radiology, University Medical Center Göttingen, Göttingen Heart Center, Robert-Koch-Str. 40, 37075, Göttingen, Germany sug: subj: Magnetic Resonance Imaging Methods Leukocytes, Mononuclear Metabolism DNA Lymphocytes Metabolism Prospective Studies Heart Male Female Flow Cytometry Methods Proteins Metabolism Randomized Controlled Trials Random Assignment Human Male Female ab: Objectives: Magnetic resonance imaging (MRI) is regarded as a non-harming and non-invasive imaging modality with high tissue contrast and almost no side effects. Compared to other cross-sectional imaging modalities, MRI does not use ionising radiation. Recently, however, strong magnetic fields as applied in clinical MRI scanners have been suspected to induce DNA double-strand breaks in human lymphocytes.Methods: In this study we investigated the impact of 3-T cardiac MRI examinations on the induction of DNA double-strand breaks in peripheral mononuclear cells by γH2AX staining and flow cytometry analysis. The study cohort consisted of 73 healthy non-smoking volunteers with 36 volunteers undergoing CMRI and 37 controls without intervention. Differences between the two cohorts were analysed by a mixed linear model with repeated measures.Results: Both cohorts showed a significant increase in the γH2AX signal from baseline to post-procedure of 6.7 % (SD 7.18 %) and 7.8 % (SD 6.61 %), respectively. However, the difference between the two groups was not significant.Conclusion: Based on our study, γH2AX flow cytometry shows no evidence that 3-T MRI examinations as used in cardiac scans impair DNA integrity in peripheral mononuclear cells.Key Points: • No evidence for DNA double-strand breaks after cardiac MRI. • Prospective study underlines safe use of MRI with regard to DNA damage. • Controlled trial involving both genders investigating DNA DSBs after 3-T MRI. pubtype: Academic Journal doctype: research tables/charts randomized controlled trial Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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